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Literature summary for 4.2.1.20 extracted from

  • Ferrari, D.; Yang, L.H.; Miles, E.W.; Dunn, M.F.
    BetaD305A mutant of tryptophan synthase shows strongly perturbed allosteric regulation and substrate specificity (2001), Biochemistry, 40, 7421-7432.
    View publication on PubMed
Search for more literature for 4.2.1.20

Protein Variants

Protein Variants Comment Organism
D305A mutation of a salt-bridging residue of the beta-subunit, substitution decreases the affinity of the beta-site for the substrate L-serine, destabilizes the enzyme-bound alpha-aminoacrylate, and quinoid species, and changes the nucleophile specificity of the beta-reaction, increased rate of pyruvate formation compared to the wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
alpha-glycerol 3-phosphate alpha-site substrate analogue, reduces the inducing effect caused by monovalent cation effectors Salmonella enterica subsp. enterica serovar Typhimurium
benzimidazole wild-type and mutant D305A enzymes Salmonella enterica subsp. enterica serovar Typhimurium
Na+ inhibits mutant D305A Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic isotope effects with alpha-2H-L-Ser, and thermodynamics, wild-type and mutant Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activates the wild-type enzyme and mutant D305A Salmonella enterica subsp. enterica serovar Typhimurium
K+ activates the wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
Na+ activates the wild-type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
NH4+ activates the wild-type enzyme and mutant D305A Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
-
 ?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
L-serine + indoline wild-type and mutant D305A enzymes Salmonella enterica subsp. enterica serovar Typhimurium dihydroiso-L-tryptophan
-
 ?
additional information aniline, phenylhydrazine and methoxylamine react with the wild-type enzyme to form quinoid reaction intermediates, which then decay, enzyme shows conformational changes between the active closed and the inactive open form during substrate recognition and binding, Asp305 of the beta-subunit plays an important role Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2 enzyme complex Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover of wild-type and mutant D305A in presence of different effectors for alpha-, and beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium
0.3
-
 1-(indol-3-yl)glycerol 3-phosphate alpha-reaction, wild-type enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.4
-
 L-serine overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
0.44
-
 1-(indol-3-yl)glycerol 3-phosphate alpha-reaction, mutant D305A enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
0.8
-
 L-serine overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of K+ Salmonella enterica subsp. enterica serovar Typhimurium
0.88
-
 L-serine beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
1.4
-
 indoline overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of 100 mM Cs+ Salmonella enterica subsp. enterica serovar Typhimurium
1.6
-
 L-serine beta-reaction, wild-type enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
2
-
 L-serine overall-reaction, wild-type enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
5
-
 L-serine overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of NH4+ Salmonella enterica subsp. enterica serovar Typhimurium
6.08
-
 L-serine beta-reaction and overall-reaction, mutant D305A enzyme, pH 7.8, 25°C Salmonella enterica subsp. enterica serovar Typhimurium
7.8
-
 L-serine overall-reaction, mutant D305A enzyme, pH 7.8, 25°C, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium
8
-
 L-serine overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of Na+ Salmonella enterica subsp. enterica serovar Typhimurium
10.3
-
 L-serine overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of NH4+ Salmonella enterica subsp. enterica serovar Typhimurium
12
-
 L-serine overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of K+ Salmonella enterica subsp. enterica serovar Typhimurium
14
-
 L-serine overall-reaction, wild-type enzyme, pH 7.8, 25°C, in presence of Cs+ Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium