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Literature summary for 4.2.1.2 extracted from

  • Kronen, M.; Berg, I.A.
    Mesaconase/Fumarase FumD in Escherichia coli O157 H7 and promiscuity of Escherichia coli class I fumarases FumA and FumB (2015), PLoS ONE, 10, e0145098 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.2

Activating Compound

Activating Compound Comment Organism Structure
thiol aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol Escherichia coli O157:H7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli
expression in Escherichia coli lysY Escherichia coli
expression in Escherichia coli lysY Escherichia coli K-12
expression in Escherichia coli lysY Escherichia coli O157:H7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.094
-
 fumarate pH 6.9, 37°C Escherichia coli
0.094
-
 fumarate 37°C, pH 6.9 Escherichia coli
0.1
-
 mesaconate pH 6.9, 37°C Escherichia coli K-12
0.1
-
 mesaconate 37°C, pH 6.9 Escherichia coli
0.15
-
 mesaconate pH 6.9, 37°C Escherichia coli O157:H7
0.15
-
 mesaconate 37°C, pH 6.9 Escherichia coli
0.2
-
 (S)-malate pH 6.9, 37°C Escherichia coli O157:H7
0.2
-
 L-malate 37°C, pH 6.9 Escherichia coli
0.21
-
 fumarate pH 6.9, 37°C Escherichia coli K-12
0.21
-
 fumarate 37°C, pH 6.9 Escherichia coli
0.22
-
 mesaconate pH 6.9, 37°C Escherichia coli
0.22
-
 mesaconate 37°C, pH 6.9 Escherichia coli
0.28
-
 fumarate pH 6.9, 37°C Escherichia coli
0.28
-
 fumarate 37°C, pH 6.9 Escherichia coli
0.4
-
 (S)-malate pH 6.9, 37°C Escherichia coli
0.4
-
 L-malate 37°C, pH 6.9 Escherichia coli
0.78
-
 (S)-malate pH 6.9, 37°C Escherichia coli K-12
0.78
-
 L-malate 37°C, pH 6.9 Escherichia coli
0.8
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli K-12
0.8
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
0.9
-
 fumarate pH 6.9, 37°C Escherichia coli O157:H7
0.9
-
 fumarate 37°C, pH 6.9 Escherichia coli
0.92
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli O157:H7
0.92
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
0.93
-
 (S)-malate pH 6.9, 37°C Escherichia coli
0.93
-
 L-malate 37°C, pH 6.9 Escherichia coli
1.04
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli K-12
1.04
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
1.08
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli
1.08
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
2
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli
2
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
2.6
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli O157:H7
2.6
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
6.2
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli
6.2
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol Escherichia coli O157:H7
additional information activity does not increase upon incubation with Fe2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 SDS-PAGE Escherichia coli
60000
-
 SDS-PAGE Escherichia coli K-12
60000
-
 SDS-PAGE Escherichia coli
60000
-
 SDS-PAGE Escherichia coli O157:H7
60105
-
 calculated from sequence Escherichia coli K-12
60105
-
 calculated from sequence Escherichia coli O157:H7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mesaconate + H2O Escherichia coli O157:H7
-
 (S)-citramalate
-
 r
mesaconate + H2O Escherichia coli O157:H7 ATCC 700728
-
 (S)-citramalate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli P05042
-
-
Escherichia coli P0AC33
-
-
Escherichia coli P14407
-
-
Escherichia coli Q8X999
-
-
Escherichia coli K-12 P14407
-
-
Escherichia coli K-12 W3110 / K-12 P14407
-
-
Escherichia coli O157:H7
-
-
-
Escherichia coli O157:H7 ATCC 700728
-
-
-
Escherichia coli W3110 / K-12 P05042
-
-
Escherichia coli W3110 / K-12 P0AC33
-
-

Oxidation Stability

Oxidation Stability Organism
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol Escherichia coli O157:H7
the enzyme remains stable under oxic conditions Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
-
 Escherichia coli K-12
-
 Escherichia coli O157:H7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-tartrate
-
 Escherichia coli oxaloacetate + H2O
-
 ?
(2S,3S)-tartrate
-
 Escherichia coli K-12 oxaloacetate + H2O
-
 ?
(2S,3S)-tartrate
-
 Escherichia coli O157:H7 oxaloacetate + H2O
-
 ?
(2S,3S)-tartrate
-
 Escherichia coli K-12 W3110 / K-12 oxaloacetate + H2O
-
 ?
(2S,3S)-tartrate
-
 Escherichia coli O157:H7 ATCC 700728 oxaloacetate + H2O
-
 ?
(S)-citramalate
-
 Escherichia coli K-12 mesaconate + H2O
-
 r
(S)-citramalate
-
 Escherichia coli mesaconate + H2O
-
 r
(S)-citramalate
-
 Escherichia coli O157:H7 mesaconate + H2O
-
 r
(S)-citramalate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli mesaconate + H2O
-
 r
(S)-citramalate
-
 Escherichia coli K-12 W3110 / K-12 mesaconate + H2O
-
 r
(S)-malate
-
 Escherichia coli fumarate + H2O
-
 r
(S)-malate
-
 Escherichia coli K-12 fumarate + H2O
-
 r
(S)-malate
-
 Escherichia coli O157:H7 fumarate + H2O
-
 r
(S)-malate the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli fumarate + H2O
-
 r
(S)-malate
-
 Escherichia coli K-12 W3110 / K-12 fumarate + H2O
-
 r
(S)-malate
-
 Escherichia coli W3110 / K-12 fumarate + H2O
-
 r
(S)-malate
-
 Escherichia coli O157:H7 ATCC 700728 fumarate + H2O
-
 r
(S,S)-tartrate
-
 Escherichia coli oxaloacetate + H2O
-
 ?
(S,S)-tartrate
-
 Escherichia coli W3110 / K-12 oxaloacetate + H2O
-
 ?
fumarate + H2O
-
 Escherichia coli L-malate
-
 r
fumarate + H2O
-
 Escherichia coli W3110 / K-12 L-malate
-
 r
fumarate + H2O
-
 Escherichia coli (S)-malate
-
 r
fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli K-12 (S)-malate
-
 r
fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli O157:H7 (S)-malate
-
 r
fumarate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli (S)-malate
-
 r
fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli K-12 W3110 / K-12 (S)-malate
-
 r
fumarate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli O157:H7 ATCC 700728 (S)-malate
-
 r
L-malate
-
 Escherichia coli fumarate + H2O
-
 r
L-malate
-
 Escherichia coli W3110 / K-12 fumarate + H2O
-
 r
mesaconate + H2O
-
 Escherichia coli (S)-citramalate
-
 r
mesaconate + H2O
-
 Escherichia coli O157:H7 (S)-citramalate
-
 r
mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli K-12 (S)-citramalate
-
 r
mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli O157:H7 (S)-citramalate
-
 r
mesaconate + H2O the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate Escherichia coli (S)-citramalate
-
 r
mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli K-12 W3110 / K-12 (S)-citramalate
-
 r
mesaconate + H2O
-
 Escherichia coli O157:H7 ATCC 700728 (S)-citramalate
-
 r
mesaconate + H2O heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate Escherichia coli O157:H7 ATCC 700728 (S)-citramalate
-
 r
additional information almost no activity with mesaconate Escherichia coli ?
-
 ?
additional information enzyme is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate Escherichia coli ?
-
 ?
additional information almost no activity with mesaconate Escherichia coli W3110 / K-12 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Escherichia coli
? x * 50000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
FumA
-
 Escherichia coli
fumarase A
-
 Escherichia coli
fumarase C
-
 Escherichia coli
FumB
-
 Escherichia coli
FumC
-
 Escherichia coli
FumD
-
 Escherichia coli K-12
FumD
-
 Escherichia coli O157:H7
mesaconase/fumarase
-
 Escherichia coli K-12
mesaconase/fumarase
-
 Escherichia coli O157:H7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
0.09437
-
 assay at Escherichia coli K-12
37
-
 assay at Escherichia coli
37
-
 assay at Escherichia coli O157:H7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 assay at Escherichia coli
6.9
-
 assay at Escherichia coli K-12
6.9
-
 assay at Escherichia coli O157:H7

General Information

General Information Comment Organism
metabolism the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various C5-dicarboxylic acids such as mesaconate or L-threo-beta-methylmalate. fumD is clustered with the key genes for two enzymes of the methylaspartate pathway of glutamate fermentation, glutamate mutase and methylaspartate ammonia lyase, converting glutamate to mesaconate Escherichia coli K-12
metabolism the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various C5-dicarboxylic acids such as mesaconate or L-threo-beta-methylmalate. fumD is clustered with the key genes for two enzymes of the methylaspartate pathway of glutamate fermentation, glutamate mutase and methylaspartate ammonia lyase, converting glutamate to mesaconate Escherichia coli O157:H7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
0.05
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli O157:H7
0.065
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
0.065
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli
0.635
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
0.635
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli
2.13
-
 (S,S)-Tartrate 37°C, pH 6.9 Escherichia coli
2.13
-
 (2S,3S)-tartrate pH 6.9, 37°C Escherichia coli K-12
35
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
35
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli
38
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
38
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli K-12
110
-
 (S)-citramalate 37°C, pH 6.9 Escherichia coli
110
-
 (S)-citramalate pH 6.9, 37°C Escherichia coli O157:H7
250
-
 mesaconate 37°C, pH 6.9 Escherichia coli
250
-
 mesaconate pH 6.9, 37°C Escherichia coli
260
-
 L-malate 37°C, pH 6.9 Escherichia coli
260
-
 (S)-malate pH 6.9, 37°C Escherichia coli O157:H7
370
-
 L-malate 37°C, pH 6.9 Escherichia coli
370
-
 (S)-malate pH 6.9, 37°C Escherichia coli K-12
390
-
 L-malate 37°C, pH 6.9 Escherichia coli
390
-
 (S)-malate pH 6.9, 37°C Escherichia coli
580
-
 mesaconate 37°C, pH 6.9 Escherichia coli
580
-
 mesaconate pH 6.9, 37°C Escherichia coli K-12
750
-
 fumarate 37°C, pH 6.9 Escherichia coli
750
-
 fumarate pH 6.9, 37°C Escherichia coli O157:H7
870
-
 L-malate 37°C, pH 6.9 Escherichia coli
870
-
 (S)-malate pH 6.9, 37°C Escherichia coli
2000
-
 mesaconate 37°C, pH 6.9 Escherichia coli
2000
-
 mesaconate pH 6.9, 37°C Escherichia coli O157:H7
3120
-
 fumarate 37°C, pH 6.9 Escherichia coli
3120
-
 fumarate pH 6.9, 37°C Escherichia coli K-12
3500
-
 fumarate 37°C, pH 6.9 Escherichia coli
3500
-
 fumarate pH 6.9, 37°C Escherichia coli
6600
-
 fumarate 37°C, pH 6.9 Escherichia coli
6600
-
 fumarate pH 6.9, 37°C Escherichia coli