Application | Comment | Organism |
---|---|---|
synthesis | use of tryptophan synthase in the generation of L-dihalotryptophans and L-alkynyltryptophans | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
A227V | 3.3-fold improvement of half-life at 50°C and a 3.6°C increase in temperature at which the activity of enzyme decreases by 50% in 15 min compared to the wild-type enzyme | Corynebacterium glutamicum |
A227V/A411V | half-life at 50°C and temperature at which the activity of enzyme decreases by 50% in 15 min increases to more than 768 min and 52.4°C, respectively | Corynebacterium glutamicum |
A227V/A411V/E175K | half-life at 50°C increases to more than 2700 min and the temperature at which the activity of enzyme decreases by 50% in 15 min is 9.8°C higher than the wild-type enzyme | Corynebacterium glutamicum |
A411V | half-life at 50°C increases from 1 min for wild-type to 2.2 min, and the temperature at which the activity of enzyme decreases by 50% in 15 min increases from 44.8°C to 47.2°C | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q8NRN8 | - |
- |
Corynebacterium glutamicum DSM 20300 | Q8NRN8 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
FumC | - |
Corynebacterium glutamicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
44.8 | - |
wild-type, 15 min, 50% residual activity | Corynebacterium glutamicum |
47.2 | - |
mutant A411V, 15 min, 50% residual activity | Corynebacterium glutamicum |
49.4 | - |
mutant A227V, 15 min, 50% residual activity | Corynebacterium glutamicum |
50 | - |
half-life of wild-type 1 min, of mutant A411V 2.2 min, of mutant A227V 4.3 min, of mutant A227V/A411V more than 768 min, of mutant A227V/A411V/E175K more than 2700 min | Corynebacterium glutamicum |
52.4 | - |
mutant A227V/A411V, 15 min, 50% residual activity | Corynebacterium glutamicum |
54.6 | - |
mutant A227V/A411V/E175K, 15 min, 50% residual activity | Corynebacterium glutamicum |