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Literature summary for 4.2.1.2 extracted from

  • Lin, L.; Wang, Y.; Wu, M.; Zhu, L.; Yang, L.; Lin, J.
    Enhancing the thermostability of fumarase C from Corynebacterium glutamicum via molecular modification (2018), Enzyme Microb. Technol., 115, 45-51 .
    View publication on PubMed

Application

Application Comment Organism
synthesis use of tryptophan synthase in the generation of L-dihalotryptophans and L-alkynyltryptophans Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
A227V 3.3-fold improvement of half-life at 50°C and a 3.6°C increase in temperature at which the activity of enzyme decreases by 50% in 15 min compared to the wild-type enzyme Corynebacterium glutamicum
A227V/A411V half-life at 50°C and temperature at which the activity of enzyme decreases by 50% in 15 min increases to more than 768 min and 52.4°C, respectively Corynebacterium glutamicum
A227V/A411V/E175K half-life at 50°C increases to more than 2700 min and the temperature at which the activity of enzyme decreases by 50% in 15 min is 9.8°C higher than the wild-type enzyme Corynebacterium glutamicum
A411V half-life at 50°C increases from 1 min for wild-type to 2.2 min, and the temperature at which the activity of enzyme decreases by 50% in 15 min increases from 44.8°C to 47.2°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q8NRN8
-
-
Corynebacterium glutamicum DSM 20300 Q8NRN8
-
-

Synonyms

Synonyms Comment Organism
FumC
-
Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
44.8
-
wild-type, 15 min, 50% residual activity Corynebacterium glutamicum
47.2
-
mutant A411V, 15 min, 50% residual activity Corynebacterium glutamicum
49.4
-
mutant A227V, 15 min, 50% residual activity Corynebacterium glutamicum
50
-
half-life of wild-type 1 min, of mutant A411V 2.2 min, of mutant A227V 4.3 min, of mutant A227V/A411V more than 768 min, of mutant A227V/A411V/E175K more than 2700 min Corynebacterium glutamicum
52.4
-
mutant A227V/A411V, 15 min, 50% residual activity Corynebacterium glutamicum
54.6
-
mutant A227V/A411V/E175K, 15 min, 50% residual activity Corynebacterium glutamicum