Application | Comment | Organism |
---|---|---|
synthesis | synthesis of fumarate in 50% ethylene glycol to shift the reaction equilibrium to fumaric acid. 54.7% conversion is observed using fumarase for transforming 1 mmol L-malic acid. 27% total yield is obtained with 99% purity | Thermus thermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethylene glycol | in presence of 70 % ethylene glycol, about 47 % L-malic acid is converted to fumaric acid, at 50 % ethylene glycol 38 % conversion occurs and at 30 % ethylene glycol 32 % conversion, respectively. After 2 days, no significant loss of activity in 50 % ethylene glycol is observed, in 70 % ethylene glycol, 80 % activity of the fumarase remain | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SKT5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
385 | - |
pH 7.8, 70°C | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Thermus thermophilus | fumarate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
FumC | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
30 min, no significant loss of activity | Thermus thermophilus |