Literature summary for 4.2.1.2 extracted from

Feliciano, P.R.; Gupta, S.; Dyszy, F.; Dias-Baruffi, M.; Costa-Filho, A.J.; Michels, P.A.; Nonato, M.C.
Fumarate hydratase isoforms of Leishmania major: subcellular localization, structural and kinetic properties (2012), Int. J. Biol. Macromol., 51, 25-31.

Search for more literature for 4.2.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as His-tagged fusion proteins	Leishmania major

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2	-	S-malate	pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 0.5 micromol/min/mg	Leishmania major
1.3	-	fumarate	pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 1.8 micromol/min/mg	Leishmania major
1.9	-	fumarate	pH 9, temperature not specified in the publication, aerobic condition, Vmax: 44.3 micromol/min/mg	Leishmania major
2.3	-	S-malate	pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 11.8 micromol/min/mg	Leishmania major
2.5	-	fumarate	pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 26.4 micromol/min/mg	Leishmania major
5.7	-	S-malate	pH 9, temperature not specified in the publication, aerobic condition, Vmax: 22.7 micromol/min/mg	Leishmania major
5.7	-	fumarate	pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 186 micromol/min/mg	Leishmania major
12.6	-	S-malate	pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 138 micromol/min/mg	Leishmania major

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Leishmania major	5829	-
glycosome	-	Leishmania major	20015	-
mitochondrion	-	Leishmania major	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
61100	-	calculated from cDNA	Leishmania major
62600	-	calculated from cDNA	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania major	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Leishmania major

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-malate	-	Leishmania major	fumarate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
LmFH-1	-	Leishmania major
LmFH-2	-	Leishmania major
LmjF24.0320	-	Leishmania major
LmjF29.1960	-	Leishmania major

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
56.3	-	melting temperature	Leishmania major
57.7	-	melting temperature	Leishmania major

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.55	-	S-malate	pH 8.7, temperature not specified in the publication, aerobic condition	Leishmania major
1.9	-	fumarate	pH 8.7, temperature not specified in the publication, aerobic condition	Leishmania major
12.9	-	S-malate	pH 8.7, temperature not specified in the publication, anaerobic condition	Leishmania major
24.9	-	S-malate	pH 9, temperature not specified in the publication, aerobic condition	Leishmania major
28.3	-	fumarate	pH 8.7, temperature not specified in the publication, anaerobic condition	Leishmania major
48.6	-	fumarate	pH 9, temperature not specified in the publication, aerobic condition	Leishmania major
151.4	-	S-malate	pH 9, temperature not specified in the publication, anaerobic condition	Leishmania major
204.2	-	fumarate	pH 9, temperature not specified in the publication, anaerobic condition	Leishmania major

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.7	-	-	Leishmania major
9	-	-	Leishmania major

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Release 2023.1 (January 2023)