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Literature summary for 4.2.1.2 extracted from

  • Regev-Rudzki, N.; Battat, E.; Goldberg, I.; Pines, O.
    Dual localization of fumarase is dependent on the integrity of the glyoxylate shunt (2009), Mol. Microbiol., 72, 297-306.
    View publication on PubMed
Search for more literature for 4.2.1.2

Cloned(Commentary)

Cloned (Comment) Organism
plasmid encoding fumarase lacking an MTS transformed into the wild-type, DELTAcit2 and R strains Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 strains BY4741 and BY4743
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
 Saccharomyces cerevisiae fumarate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FUM1
-
 Saccharomyces cerevisiae
fumarase
-
 Saccharomyces cerevisiae

Expression

Organism Comment Expression
Saccharomyces cerevisiae level of fumarase mRNA is upregulated both in the DELTacit2 and wild-type plus pCit2 strains. Approximately 2fold higher Fum1 mRNA level in a DELTAcit2 strain when compared with the wild-type strain. Significant increase in fumarase expression and cellular enzymatic activity in the glyoxylate deletion strains: DELTAmls1, DELTAicl1, DELTAaco1and DELTAcit2. Succinic acid in the growth medium affects fumarase dual distribution, succinic acid directly interacts with fumarase and slows down its folding thereby causing more fumarase to be fully imported into mitochondria up

General Information

General Information Comment Organism
physiological function metabolites of the glyoxylate shunt act as nanosensors for fumarase subcellular targeting and distribution. Glyoxylate shunt deletion mutants exhibit an altered fumarase dual distribution. Expression levels of Cit2 affect dual targeting of fumarase. Amount of cytosolic fumarase is drastically reduced in the DELTAcit2 strain, when compared with the wild-type strain. Proportion of fumarase activity is very low in the cytosolic versus mitochondrial fractions obtained from DELTAcit2 and wild-type plus pCit2 strains when compared with the wild-type or DELTAcit2 plus pCit2 strains, in which the fumarase activity is divided equally between the corresponding subcellular fractions Saccharomyces cerevisiae