Literature summary for 4.2.1.2 extracted from

Kulkarni, G.; Sabnis, N.A.; Harris, B.G.
Cloning, expression, and purification of fumarase from the parasitic nematode Ascaris suum (2004), Protein Expr. Purif., 33, 209-213.

Search for more literature for 4.2.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Ascaris suum

Inhibitors

Inhibitors	Comment	Organism	Structure
mesotartaric acid	competitive	Ascaris suum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.22	-	(S)-malate	pH 7.3, 30°C, recombinant enzyme	Ascaris suum
1.43	-	(S)-malate	pH 7.3, 30°C, worm enzyme	Ascaris suum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50502	-	x * 50502, calculation from nucleotide sequence	Ascaris suum
51000	-	x * 51000, SDS-PAGE	Ascaris suum

Organism

Organism	UniProt	Comment	Textmining
Ascaris suum	Q7Z1D9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Ascaris suum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
388	-	-	Ascaris suum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate	-	Ascaris suum	fumarate + H2O	-	r
fumarate + H2O	-	Ascaris suum	L-malate	-	?

Subunits

Subunits	Comment	Organism
?	x * 51000, SDS-PAGE	Ascaris suum
?	x * 50502, calculation from nucleotide sequence	Ascaris suum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.077	-	mesotartaric acid	pH 7.3, 30°C	Ascaris suum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)