Protein Variants | Comment | Organism |
---|---|---|
H129N | loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 3.1fold reduced maximal velococity in reaction with malate, 1.13fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme | Escherichia coli |
K127D | mutant enzyme behaves like wild-type enzyme | Escherichia coli |
R126A | loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 4.3fold reduced maximal velococity in reaction with malate, 2.7fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme | Escherichia coli |
R126A/H129N | great loss of D2O inhibitory effect, product release step is accelerated by glycerol compared to inhibition of wild-type enzyme. 8.6fold reduced maximal velococity in reaction with malate, 7.1fold reduced maximal velocity in reaction with fumarate compared to wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | wild-type enzyme is inhibited due to a viscogenic effect on the recycling rate | Escherichia coli | |
meso-tartrate | - |
Escherichia coli | |
additional information | inhibition by D2O arises in the recycling phase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
FumC | - |
Escherichia coli FumC | - |
FumC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Escherichia coli | fumarate + H2O | wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release | ? | |
(S)-malate | - |
Escherichia coli FumC | fumarate + H2O | wild-type enzyme is rate-limited in the recycling of free enzyme isoforms that follows product release | ? |
Synonyms | Comment | Organism |
---|---|---|
FumC | - |
Escherichia coli |