General Stability | Organism |
---|---|
50% v/v ethanol, 50% v/v methanol, 50% v/v 2-propanol, 4 M urea or 0.1% SDS, stable after treatment for 10 h at room temperature | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | 15-20% of maximum activity when assayed in 40% (v/v) 2-propanol | Saccharolobus solfataricus | |
ethanol | 15-20% of maximum activity when assayed in 40% (v/v) ethanol | Saccharolobus solfataricus | |
K3PO4 | 10 mM | Saccharolobus solfataricus | |
methanol | 94% of maximum activity when assayed in 40% (v/v) methanol | Saccharolobus solfataricus | |
phosphate | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
fumarate | - |
Saccharolobus solfataricus | |
0.125 | - |
fumarate | pH 7.5, 70°C | Saccharolobus solfataricus | |
0.3 | - |
(S)-malate | - |
Saccharolobus solfataricus | |
0.3 | - |
(S)-malate | pH 7.5, 70°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Saccharolobus solfataricus |
160000 | - |
sucrose density gradient centrifugation | Saccharolobus solfataricus |
170000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days | Saccharolobus solfataricus |
Ethanol | 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days | Saccharolobus solfataricus |
Methanol | 50% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days | Saccharolobus solfataricus |
SDS | 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days | Saccharolobus solfataricus |
urea | 0.1% (v/v), 10 h at room temperature, no significant loss of activity, slight loss of activity after 14 days | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | - |
DSM 1616 | - |
Saccharolobus solfataricus DSM 1616 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
205 | - |
- |
Saccharolobus solfataricus |
205 | - |
pH 7.5, 70°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Saccharolobus solfataricus | fumarate + H2O | - |
r | |
(S)-malate | - |
Saccharolobus solfataricus DSM 1616 | fumarate + H2O | - |
r | |
fumarate + H2O | - |
Saccharolobus solfataricus | L-malate | - |
r | |
fumarate + H2O | - |
Saccharolobus solfataricus DSM 1616 | L-malate | - |
r | |
additional information | no activity with D-malate, maleate, citramalate and citrate | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity with D-malate, maleate, citramalate and citrate | Saccharolobus solfataricus DSM 1616 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
85 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 95 | inactive at 40°C, 85°C: optimum, 95°C: 66% of maximal activity | Saccharolobus solfataricus |
85 | 95 | 85°C: maximal activity, 95°C: 66% of maximal activity, the enzyme is almost inactive at 40°C | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, stable | Saccharolobus solfataricus |
85 | - |
1 h, stable | Saccharolobus solfataricus |
90 | - |
30 min, 72% of maximal activity | Saccharolobus solfataricus |
90 | - |
30 min, 72% loss of activity | Saccharolobus solfataricus |
100 | - |
30 min, complete loss of activity | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |
8 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: 43% of maximal activity, pH 9.0: 70% of maximal activity | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
7.1 |