Activating Compound | Comment | Organism | Structure |
---|---|---|---|
arsenate | activates | Sus scrofa | |
Borate | activates | Sus scrofa | |
citrate | activates | Sus scrofa | |
phosphate | increases activity at less than 5 mM, competitive inhibition at higher concentrations | Sus scrofa | |
selenate | activates | Sus scrofa | |
SO42- | activates | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-bromocrotonate | - |
Sus scrofa | |
ATP | - |
Sus scrofa | |
bromofumarate | - |
Sus scrofa | |
chlorofumarate | - |
Sus scrofa | |
citrate | - |
Sus scrofa | |
CTP | - |
Sus scrofa | |
dimethylfumarate | - |
Sus scrofa | |
GTP | - |
Sus scrofa | |
iodoacetamide | - |
Sus scrofa | |
iodoacetate | - |
Sus scrofa | |
iodofumarate | - |
Sus scrofa | |
isocitrate | L-isocitrate | Sus scrofa | |
L-Tartrate | - |
Sus scrofa | |
L-threo-hydroxyaspartate | - |
Sus scrofa | |
mesaconate | - |
Sus scrofa | |
additional information | substrate inhibition between 0.1 M and 1.0 M | Sus scrofa | |
NEM | - |
Sus scrofa | |
phosphate | increases activity at less than 5 mM, competitive inhibition at higher concentrations | Sus scrofa | |
trans-aconitate | - |
Sus scrofa | |
UTP | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
fumarate | - |
Sus scrofa | |
0.02 | 0.14 | L-threo-chloromalate | - |
Sus scrofa | |
0.025 | - |
L-malate | - |
Sus scrofa | |
0.027 | - |
fluorofumarate | - |
Sus scrofa | |
0.11 | - |
chlorofumarate | - |
Sus scrofa | |
0.11 | - |
bromofumarate | - |
Sus scrofa | |
0.12 | - |
iodofumarate | - |
Sus scrofa | |
0.145 | - |
acetylene dicarboxylate | - |
Sus scrofa | |
0.51 | - |
mesaconate | - |
Sus scrofa | |
1.3 | - |
L-Tartrate | - |
Sus scrofa | |
7.5 | 10 | L-threo-hydroxyaspartate | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48500 | - |
4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25 | Sus scrofa |
194000 | - |
equilibrium sedimentation | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
several isoenzymes | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-malate = fumarate + H2O | mechanism | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Sus scrofa | fumarate + H2O | - |
r | |
acetylene dicarboxylate + H2O | - |
Sus scrofa | oxaloacetate | - |
? | |
alpha-fluorofumarate + H2O | - |
Sus scrofa | oxaloacetate + ? | - |
? | |
bromofumarate + H2O | - |
Sus scrofa | ? | - |
? | |
chlorofumarate + H2O | - |
Sus scrofa | ? | - |
? | |
fluorofumarate + H2O | - |
Sus scrofa | ? | - |
? | |
fumarate + H2O | - |
Sus scrofa | L-malate | - |
r | |
iodofumarate + H2O | - |
Sus scrofa | ? | - |
? | |
L-tartrate | - |
Sus scrofa | oxaloacetate + H2O | - |
? | |
L-threo-chloro-L-malate | - |
Sus scrofa | chlorofumarate + H2O | - |
? | |
L-threo-hydroxyaspartate | - |
Sus scrofa | ? | - |
? | |
mesaconate + H2O | - |
Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25 | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at low concentrations, phosphate, sulfate, borate, selenate, arsenate and citrate activate fumarase and shift somewhat the pH optimum to a more alkaline pH | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | stable in potassium phosphate buffer, above pH 10: dissociation into an inactive form | Sus scrofa |
6 | 10 | dissociation below pH 6 and above pH 10, Tris-acetate buffer | Sus scrofa |