BRENDA - Enzyme Database show
show all sequences of 4.2.1.19

Purification and characterization of the imidazoleglycerol-phosphate dehydratase of Saccharomyces cerevisiae from recombinant Escherichia coli

Hawkes, T.R.; Thomas, P.G.; Edwards, L.S.; Rayner, S.J.; Wilkinson, K.W.; Biochem. J. 306, 385-397 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-Amino-1,2,4-triazole
Ki: 0.12 mM
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
essential for assembly of subunits
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23833
-
x * 23833, light scattering
Saccharomyces cerevisiae
70000
-
trimeric enzyme in absence of Mn2+, gel filtration
Saccharomyces cerevisiae
500000
-
polymeric enzyme in presence of Mn2+, gel filtration
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
5546
Saccharomyces cerevisiae
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
-
-
-
Subunits
Subunits
Commentary
Organism
polymer
x * 23833, light scattering
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-Amino-1,2,4-triazole
Ki: 0.12 mM
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
essential for assembly of subunits
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23833
-
x * 23833, light scattering
Saccharomyces cerevisiae
70000
-
trimeric enzyme in absence of Mn2+, gel filtration
Saccharomyces cerevisiae
500000
-
polymeric enzyme in presence of Mn2+, gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
5546
Saccharomyces cerevisiae
3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
polymer
x * 23833, light scattering
Saccharomyces cerevisiae
Other publictions for EC 4.2.1.19
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749384
Bisson
Crystal structures reveal tha ...
Arabidopsis thaliana
Structure
23
1236-1245
2015
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728872
Ahangar
Structures of native, substrat ...
Mycobacterium tuberculosis
Acta Crystallogr. Sect. D
69
2461-2467
2013
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730174
Park
Cloning and functional verific ...
Kazachstania humilis, Kazachstania humilis CBS 8195
J. Microbiol. Biotechnol.
22
1441-1445
2012
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663551
Glynn
Purification, crystallization ...
Arabidopsis thaliana
Acta Crystallogr. Sect. F
F61
776-778
2005
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1
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666944
Glynn
Structure and mechanism of imi ...
Arabidopsis thaliana
Structure
13
1809-1817
2005
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652527
Sinha
Crystal structure of imidazole ...
Cryptococcus neoformans
J. Biol. Chem.
279
15491-15498
2004
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5543
Wilkinson
Crystallization and analysis o ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
51
845-847
1995
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5544
Parker
-
Escherichia coli imidazoleglyc ...
Escherichia coli
J. Am. Chem. Soc.
117
10605-10613
1995
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5545
Tada
Insect cell expression of reco ...
Arabidopsis thaliana, Triticum aestivum
Plant Physiol.
109
153-159
1995
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2
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5
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5546
Hawkes
Purification and characterizat ...
Saccharomyces cerevisiae
Biochem. J.
306
385-397
1995
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1
1
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1
3
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3
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5547
Mori
A novel class of herbicides. S ...
Triticum aestivum
Plant Physiol.
107
719-723
1995
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5541
Parker
Cloning, sequencing and expres ...
Cryptococcus neoformans
Gene
145
135-138
1994
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5542
Tada
Isolation and characterization ...
Arabidopsis thaliana
Plant Physiol.
105
579-583
1994
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5540
Mano
Purification and properties of ...
Brassica oleracea, Hordeum vulgare, Oryza sativa, Rosa sp., Triticum aestivum, Zea mays
Plant Physiol.
103
733-739
1993
-
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3
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8
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5539
Goldman
Molecular cloning of the imida ...
Trichoderma harzianum
Mol. Gen. Genet.
234
481-488
1992
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5530
Chiariotti
Gene strucutre in the histidin ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Mol. Gen. Genet.
202
42-47
1986
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5531
Staples
Purification of the bifunction ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochim. Biophys. Acta
613
210-219
1980
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5532
Struhl
A physiological study of funct ...
Saccharomyces cerevisiae
J. Mol. Biol.
136
291-307
1980
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5537
Staples
Proteolytic degradation of imi ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
254
1395-1401
1979
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5533
Struhl
Production of a functional euk ...
Saccharomyces cerevisiae
Proc. Natl. Acad. Sci. USA
74
5255-5259
1977
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5534
Glaser
Subunit structure and photooxi ...
Saccharomyces cerevisiae
Biochemistry
13
5145-5152
1974
3
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1
2
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3
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1
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1
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5535
Brady
Some properties of the catalyt ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
248
2588-2592
1973
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3
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5536
Burke
Histidine biosynthetic pathway ...
Salmonella enterica subsp. enterica serovar Typhimurium, Staphylococcus aureus
Can. J. Microbiol.
18
569-576
1972
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5538
Ames
The biosynthesis of histidine: ...
Neurospora crassa
J. Biol. Chem.
228
131-143
1957
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1
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1
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1
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1
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1
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1
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2
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1
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