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Literature summary for 4.2.1.18 extracted from

  • Mack, M.; Liesert, M.; Zschocke, J.; Peters, V.; Linder, D.; Buckel, W.
    3-Methylglutaconyl-CoA hydratase from Acinetobacter sp (2006), Arch. Microbiol., 185, 297-306.
    View publication on PubMed
Search for more literature for 4.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, amino acid sequence determined, membership to the crotonase/isomerase super family classified by comparing protein seqences, up to 50% identity to amino acid sequences of more than 100 prokaryotic enoyl-CoA hydratases, 30% homology to the gene product of human AUH gene Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information addition of water to the (E)-isomer of 3-methylglutaconyl-CoA of the leucine degradative pathway, intermediate is (S)-3-hydroxy-3-methylglutaryl-CoA Acinetobacter sp.
0.01
-
 3-methylglutaconyl-CoA
-
 Acinetobacter sp.
0.03
-
 (S)-3-hydroxy-3-methylglutaryl-CoA
-
 Acinetobacter sp.
0.04
-
 (E)-glutaconyl-CoA hydration Acinetobacter sp.
0.05
-
 (S)-3-hydroxyglutaryl-CoA dehydration Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information stimulation by divalent metal ions such as Mg2+ not identified Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28680
-
 deduced from amino acid sequence Acinetobacter sp.
28800
-
 MALDI-TOF Acinetobacter sp.
29000
-
 SDS-PAGE Acinetobacter sp.
109000
-
 gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA Acinetobacter sp. isovalerate as sole carbon and energy source in Acinetobacter is metabolized via isovaleryl-CoA, oxidative (S)-leucine degradation pathway trans-3-methylglutaconyl-CoA + H2O
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA Acinetobacter sp. DSM 11652 isovalerate as sole carbon and energy source in Acinetobacter is metabolized via isovaleryl-CoA, oxidative (S)-leucine degradation pathway trans-3-methylglutaconyl-CoA + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. Q3HW12 DSMZ 11652, strain IVS-B, formerly classified as Mycobacterium IVS-B
-
Acinetobacter sp. DSM 11652 Q3HW12 DSMZ 11652, strain IVS-B, formerly classified as Mycobacterium IVS-B
-

Purification (Commentary)

Purification (Comment) Organism
from cell-free extracts of isovalerate-grown cells of Acinetobacter strain IVS-B by gel-filtration and SDS-PAGE Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay in presence of 0.5 ml 50 mM potassium phosphate pH 7.0 and 0.1 mM glutaconyl-CoA, kinetic constants determined by HPLC, 3-methylated CoA-thiol esters identified as the real substrates as expected from the pathway of isovalerate oxidation, specificity constants of other substrates as glutaconyl-CoA and 3-hydroxyglutaryl-CoA are 22 and 7 times lower, no activities measured for crotonyl-CoA Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-glutaconyl-CoA
-
 Acinetobacter sp. ?
-
 ?
(E)-glutaconyl-CoA
-
 Acinetobacter sp. DSM 11652 ?
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA
-
 Acinetobacter sp. trans-3-methylglutaconyl-CoA + H2O
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA isovalerate as sole carbon and energy source in Acinetobacter is metabolized via isovaleryl-CoA, oxidative (S)-leucine degradation pathway Acinetobacter sp. trans-3-methylglutaconyl-CoA + H2O
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA
-
 Acinetobacter sp. DSM 11652 trans-3-methylglutaconyl-CoA + H2O
-
 ?
(S)-3-hydroxy-3-methylglutaryl-CoA isovalerate as sole carbon and energy source in Acinetobacter is metabolized via isovaleryl-CoA, oxidative (S)-leucine degradation pathway Acinetobacter sp. DSM 11652 trans-3-methylglutaconyl-CoA + H2O
-
 ?
(S)-3-hydroxyglutaryl-CoA
-
 Acinetobacter sp. ?
-
 ?
(S)-3-hydroxyglutaryl-CoA
-
 Acinetobacter sp. DSM 11652 ?
-
 ?
3-methylglutaconyl-CoA
-
 Acinetobacter sp. ?
-
 ?
3-methylglutaconyl-CoA
-
 Acinetobacter sp. DSM 11652 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 28800, four identical subunits, determined by SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
3-methylglutaconyl-CoA hydratase
-
 Acinetobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information calculated with the subunit molecular mass of 3-methylglutaconyl-CoA hydratase of 28800 Dalton Acinetobacter sp.
13
-
 (S)-3-hydroxyglutaryl-CoA
-
 Acinetobacter sp.
18
-
 (E)-glutaconyl-CoA
-
 Acinetobacter sp.
60
-
 (S)-3-hydroxy-3-methylglutaryl-CoA
-
 Acinetobacter sp.
138
-
 3-methylglutaconyl-CoA
-
 Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
additional information cofactors not identified in the purified enzyme fraction Acinetobacter sp.