Literature summary for 4.2.1.168 extracted from

Cook, P.; Holden, H.
GDP-4-keto-6-deoxy-D-mannose 3-dehydratase, accommodating a sugar substrate in the active site (2008), J. Biol. Chem., 283, 4295-4303.

Search for more literature for 4.2.1.168

Crystallization (Commentary)

Crystallization (Comment)	Organism
H188N mutant with bound GDP-perosamine, to 1.7 A resolution. The sugar analog is trapped in the active site as an external aldimine. The active site is positioned between the two subunits of the dimer. The diphosphoryl groups of the ligand are anchored to the protein via Arg219 and Arg331, the hydroxyl groups of the hexose only lie within hydrogen bonding distance to ordered water molecules. The hexose moiety of the ligand adopts a boat rather than the typically observed chair conformation	Escherichia coli

Crystallization (Comment)

Organism

H188N mutant with bound GDP-perosamine, to 1.7 A resolution. The sugar analog is trapped in the active site as an external aldimine. The active site is positioned between the two subunits of the dimer. The diphosphoryl groups of the ligand are anchored to the protein via Arg219 and Arg331, the hydroxyl groups of the hexose only lie within hydrogen bonding distance to ordered water molecules. The hexose moiety of the ligand adopts a boat rather than the typically observed chair conformation

Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
H188K	mutant protein cannot catalyze the dehydration step	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q9F118	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-4-dehydro-alpha-D-rhamnose + L-glutamate	-	Escherichia coli	GDP-4-dehydro-3,6-dideoxy-alpha-D-mannose + 2-oxoglutarate + ammonia	-	?
GDP-D-perosamine + L-glutamate	-	Escherichia coli	GDP-4-dehydro-3,6-dideoxymannose + 2-oxoglutarate + ammonia	wild-type ColD is able to catalyze the production of GDP-4-keto-3,6-dideoxymannose using GDP-perosamine instead of GDP-4-keto-6-deoxymannose as a substrate	?

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Release 2023.1 (January 2023)