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Literature summary for 4.2.1.167 extracted from

  • Hans, M.; Sievers, J.; Müller, U.; Bill, E.; Vorholt, J.A.; Linder, D.; Buckel, W.
    2-Hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum (1999), Eur. J. Biochem., 265, 404-414.
    View publication on PubMed
Search for more literature for 4.2.1.167

Activating Compound

Activating Compound Comment Organism Structure
(R)-2-hydroxyglutaryl-CoA dehydratase component A
-
 [Clostridium] symbiosum
FMNH2 1 mol per mol heterodimeric enzyme [Clostridium] symbiosum
riboflavin 0.3 mol per mol heterodimeric enzyme [Clostridium] symbiosum

Cloned(Commentary)

Cloned (Comment) Organism
-
 [Clostridium] symbiosum

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S centre the enzyme contains two [4Fe4S]2+ clusters. It is likely that the alpha and beta subunits are bridged by a [4Fe4S]2+ cluster. The enzyme contains 7.5 non-heme iron and 8.1 acid labile sulfur/heterodimer, symmetric cube-type structures [Clostridium] symbiosum
[4Fe-4S]2+ cluster 7.3 mol iron per mol heterodimeric enzyme, 8.1 mol sulfur per mol heterodimeric enzyme [Clostridium] symbiosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 [Clostridium] symbiosum
42000
-
 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
54000
-
-
 [Clostridium] symbiosum
54000
-
 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
100000
-
 gel filtration [Clostridium] symbiosum

Organism

Organism UniProt Comment Textmining
[Clostridium] symbiosum Q9X5B7 and Q9X5B8
-
-
[Clostridium] symbiosum Q9X5B8 AND Q9X5B7 Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA)
-
[Clostridium] symbiosum DSM 934 Q9X5B7 and Q9X5B8
-
-
[Clostridium] symbiosum HB 25 Q9X5B8 AND Q9X5B7 Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA)
-

Oxidation Stability

Oxidation Stability Organism
oxygen sensitive with a half-life of 2 days under aerobic conditions [Clostridium] symbiosum
the enzyme is oxygen sensitive with a half-life of 2 days under aerobic conditions [Clostridium] symbiosum
under anaerobic conditions at 4°C for about 1 week without loss of activity [Clostridium] symbiosum

Purification (Commentary)

Purification (Comment) Organism
-
 [Clostridium] symbiosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32
-
 strictly anaerobic conditions, substrate (R)-2-hydroxyglutaryl-CoA, 200 mM potassium phosphate pH 7.0, 22°C [Clostridium] symbiosum

Storage Stability

Storage Stability Organism
-80°C, frozen with liquid nitrogen, stable [Clostridium] symbiosum
-80°C, liquid nitrogen, long-term storage [Clostridium] symbiosum
4°C anaerobic conditions, 1 week stable [Clostridium] symbiosum
4°C, 1 week, anaerobic conditions, stable [Clostridium] symbiosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-hydroxyglutaryl-CoA
-
 [Clostridium] symbiosum (E)-glutaconyl-CoA + H2O
-
 r
(R)-2-hydroxyglutaryl-CoA
-
 [Clostridium] symbiosum DSM 934 (E)-glutaconyl-CoA + H2O
-
 r

Subunits

Subunits Comment Organism
heterodimer 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
heterodimer 1 * 54000 (alpha) + 1 * 42000 (beta), SDS-PAGE [Clostridium] symbiosum

Synonyms

Synonyms Comment Organism
hgdAB
-
 [Clostridium] symbiosum

Cofactor

Cofactor Comment Organism Structure
riboflavin 5'-phosphate the enzyme contains 1.0 mol of riboflavin 5'-phosphate per mol of heterodimeric enzyme [Clostridium] symbiosum