Any feedback?
Literature summary for 4.2.1.149 extracted from
- Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli (2001), Biochemistry, 40, 11140-11148.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
crotonobetainyl-CoA | at pH 7.5 and 37°C | Escherichia coli |  |
| 0.038 | - |
crotonyl-CoA | at pH 7.5 and 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
| 87000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonobetainyl-CoA + H2O | Escherichia coli | - |
L-carnitinyl-CoA | - |
r | |
| crotonyl-CoA + H2O | Escherichia coli | - |
? | - |
? | |
| L-carnitinyl-CoA | Escherichia coli | - |
crotonobetainyl-CoA + H2O | - |
r | |
| additional information | Escherichia coli | does not use crotonobetaine as substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P31551 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose column chromatography and Resource Q column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60.3 | - |
cell-free extract, at pH 7.5 and 37°C | Escherichia coli |
| 699.9 | - |
after 11.6fold purification, at pH 7.5 and 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotonobetainyl-CoA + H2O | - |
Escherichia coli | L-carnitinyl-CoA | - |
r | |
| crotonyl-CoA + H2O | - |
Escherichia coli | ? | - |
? | |
| L-carnitinyl-CoA | - |
Escherichia coli | crotonobetainyl-CoA + H2O | - |
r | |
| additional information | does not use crotonobetaine as substrate | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CaiD | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
