Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Methylorubrum extorquens |
Crystallization (Comment) | Organism |
---|---|
non-labeled and selenomethionine-labeled enzyme, hanging drop vapor diffusion method, using 0.2 M calcium chloride hydrate, 0.1 M sodium acetate hydrate, pH 4.6, and 10-20% (v/v) isopropanol | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
5,6,7,8-tetrahydromethanopterin + formaldehyde | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
5,10-methylenetetrahydromethanopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | Q9FA38 | - |
- |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | Q9FA38 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens | 5,10-methylenetetrahydromethanopterin + H2O | - |
? | |
5,6,7,8-tetrahydromethanopterin + formaldehyde | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,10-methylenetetrahydromethanopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homopentamer | x-ray crystallography | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
FAE | - |
Methylorubrum extorquens |
formaldehyde-activating enzyme | - |
Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydromethanopterin | dependent on | Methylorubrum extorquens |
General Information | Comment | Organism |
---|---|---|
physiological function | formaldehyde-activating enzyme is essential for energy metabolism and formaldehyde detoxification | Methylorubrum extorquens |