Cloned (Comment) | Organism |
---|---|
transient expression of GFP-tagged enzyme in Nicotiana tabacum BY-2 cells, expression of N-terminally His-tagged mature dehydratase in Escherichia coli strain BL21 (DE3) RIPL | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme knockout lines using T-DNA insertion, phenotype, overview | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00069 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
0.00086 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
0.00089 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
0.00115 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
chloroplast | - |
Zea mays | 9507 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
cytosol | - |
Zea mays | 5829 | - |
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
mitochondrion | - |
Zea mays | 5739 | - |
additional information | the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX | Arabidopsis thaliana | - |
- |
additional information | the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX | Zea mays | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana | |
Mg2+ | required | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Zea mays | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Columbia-0 | - |
ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana | - |
ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Zea mays | - |
ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Columbia-0 | - |
ADP + phosphate + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94AF2 | - |
- |
Arabidopsis thaliana Columbia-0 | Q94AF2 | - |
- |
Zea mays | A0A3L6F337 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged mature dehydratase from Escherichia coli strain BL21 (DE3) RIPL by nickel affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Zea mays | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Zea mays | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Zea mays | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADPH | - |
? | |
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)HX dehydratase | - |
Arabidopsis thaliana |
NAD(P)HX dehydratase | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arabidopsis thaliana |
22 | - |
assay at | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
0.3 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
0.34 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
0.38 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
8 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | dependent on | Arabidopsis thaliana | |
ADP | dependent on | Zea mays |
General Information | Comment | Organism |
---|---|---|
malfunction | ablation of the Arabidopsis dehydratase gene raises seedling levels of all NADHX forms by 20 to 40fold, and levels of one NADPHX form by 10 to 30fold | Arabidopsis thaliana |
metabolism | plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Arabidopsis thaliana |
metabolism | plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Zea mays |
physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner | Arabidopsis thaliana |
physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner | Zea mays |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
262 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
358 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
509 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
524 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays |