Cloned (Comment) | Organism |
---|---|
gene Pdeg or pglF, recombinant expression in Escherichia coli, coexpression with Preq (UDP-4-reductase), UDP-GlcNAc 6-DH (UDP-N-acetylglucosamine 6-dehydrogenase), and UXNAcS (UDP-N-acetylxylosamine synthase), leading to biosynthesis of flavonoid glycosides in the engineered Escherichia coli strain, overview | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | four genes, Pdeg (UDP-N-acetylglucosamine C4,6-dehydratase), Preq (UDP-4-reductase), UDP-GlcNAc 6-DH (UDP-N-acetylglucosamine 6-dehydrogenase), and UXNAcS (UDP-N-acetylxylosamine synthase), are employed to synthesize UDP-quinovosamine, UDP-N-acetylglucosaminuronic acid, and UDP-N-acetylxylosamine in Escherichia coli. Escherichia coli nucleotide sugar biosynthetic pathway is engineered to increase the pool of substrate for the target nucleotide sugars. Uridine diphosphate dependent glycosyltransferase (UGT) is also selected and introduced into Escherichia coli. High levels of three novel flavonoid glycosides are obtained in the engineered mutant with 158.3 mg/l quercetin 3-O-(N-acetyl) quinovosamine, 172.5 mg/l luteolin 7-O-(N-acetyl)glucosaminuronic acid, and 160.8 mg/l quercetin 3-O-(N-acetyl)xylosamine | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | - |
UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus | Q81A42 | - |
- |
Bacillus cereus ATCC 14579 | - |
- |
- |
Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | Q81A42 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus ATCC 14579 | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine | - |
Bacillus cereus ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711 | UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BC_3750 | - |
Bacillus cereus |
Pdeg | - |
Bacillus cereus |
UDP-N-acetylglucosamine C4,6-dehydratase | - |
Bacillus cereus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzme is involved in the biosynthesis of Bacillus glycans and N-acetylquinovosamine (2-acetamido-2,6-di-deoxy-D-glucose, QuiNAc) | Bacillus cereus |
physiological function | gene Pdeg encodes an UDP-N-acetylglucosamine C4,6-dehydratase that converts UDP-GlcNAc to UDP-4-keto-4,6-D-deoxy-GlcNAc (UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose). The product is then catalyzed by Preq-encoded UDP-4-reductase that converts UDP-4-keto-4,6-d-deoxy-GlcNAc to UDP-N-acetylquinovosamine | Bacillus cereus |