Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | at 0.04% 30fold higher activity than at 1% | Campylobacter jejuni |
Application | Comment | Organism |
---|---|---|
biotechnology | assay targets enzymes involved in the biosynthesis of the unusual bacterial sugar diNAcBac and the transfer of diNAcBac-phosphate to UndP. This multienzyme assay, together with the established assays for the individual enzymes, can be used to screen for inhibitors, and may be used to evaluate substrate flux along the inhibited pathway. This assay is optimized for maximum sensitivity to inhibition of PglF, PglE, PglD, and PglC by balancing the enzyme concentrations such that each is partially rate determining | Campylobacter jejuni |
Cloned (Comment) | Organism |
---|---|
recombinantly expressed | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylglucosamine | - |
Campylobacter jejuni | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PglF | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Campylobacter jejuni |