BRENDA - Enzyme Database show
show all sequences of 4.2.1.134

Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis

Ikeda, M.; Kanao, Y.; Yamanaka, M.; Sakuraba, H.; Mizutani, Y.; Igarashi, Y.; Kihara, A.; FEBS Lett. 582, 2435-2440 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain SAY32
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0068
-
3-hydroxypalmitoyl-CoA
isoform HCAD4, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.0336
-
3-hydroxypalmitoyl-CoA
isoform HCAD1, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.0495
-
3-hydroxypalmitoyl-CoA
isoform HCAD3, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.1217
-
3-hydroxypalmitoyl-CoA
isoform HCAD2, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54900
-
x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence
Homo sapiens
55800
-
x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence
Homo sapiens
59800
-
x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence
Homo sapiens
70600
-
x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypalmitoyl-CoA
-
719491
Homo sapiens
2,3-trans-hexadecenoyl-CoA + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence; x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence; x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence; x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain SAY32
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0068
-
3-hydroxypalmitoyl-CoA
isoform HCAD4, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.0336
-
3-hydroxypalmitoyl-CoA
isoform HCAD1, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.0495
-
3-hydroxypalmitoyl-CoA
isoform HCAD3, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
0.1217
-
3-hydroxypalmitoyl-CoA
isoform HCAD2, in 50 mM HEPES-NaOH (pH 7.4), 150 mM NaCl, 10% (v/v) glycerol, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, at 37C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Homo sapiens
5783
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54900
-
x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence
Homo sapiens
55800
-
x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence
Homo sapiens
59800
-
x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence
Homo sapiens
70600
-
x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypalmitoyl-CoA
-
719491
Homo sapiens
2,3-trans-hexadecenoyl-CoA + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54900, EGFP-tagged HACD4 isoform, estimated from amino acid sequence; x * 55800, EGFP-tagged HACD2 isoform, estimated from amino acid sequence; x * 59800, EGFP-tagged HACD1 isoform, estimated from amino acid sequence; x * 70600, EGFP-tagged HACD3 isoform, estimated from amino acid sequence
Homo sapiens
Other publictions for EC 4.2.1.134
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748533
Liang
Transcriptomic profiling and ...
Aurantiochytrium sp., Aurantiochytrium sp. PKUSW7
Mar. Drugs
16
310-324
2018
-
1
1
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1
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2
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4
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2
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1
1
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-
1
-
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-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
749092
Morineau
Dual fatty acid elongase comp ...
Arabidopsis thaliana, Saccharomyces cerevisiae
PLoS ONE
11
e0160631
2016
-
-
1
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
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6
-
-
-
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-
-
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-
-
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-
-
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2
-
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-
-
-
-
-
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1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
5
8
-
-
-
749134
Ianiri
Phs1 and the synthesis of ver ...
Sporobolomyces sp. (in: Microbotryomycetes), Sporobolomyces sp. (in: Microbotryomycetes) IAM13481
PLoS ONE
9
e105147
2014
-
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1
-
1
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-
-
-
-
-
-
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8
-
-
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-
-
-
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1
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1
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1
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-
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-
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-
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1
-
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-
-
-
-
-
-
2
2
-
-
-
720091
Bach
Very-long-chain fatty acids ar ...
Arabidopsis thaliana
J. Cell Sci.
124
3223-3234
2011
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719491
Ikeda
Characterization of four mamma ...
Homo sapiens
FEBS Lett.
582
2435-2440
2008
-
-
1
-
-
-
-
4
1
-
4
-
-
4
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
1
-
4
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719847
Kihara
Membrane topology and essentia ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
11199-11209
2008
-
-
-
-
21
-
-
-
1
-
-
1
-
3
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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21
-
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-
-
-
1
-
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1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
720893
Bach
The very-long-chain hydroxy fa ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
105
14727-14731
2008
-
-
1
-
-
-
-
-
1
-
-
-
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4
-
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-
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-
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1
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1
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1
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1
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2
2
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