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Literature summary for 4.2.1.129 extracted from

  • Sato, T.; Kouda, M.; Hoshino, T.
    Site-directed mutagenesis experiments on the putative deprotonation site of squalene-hopene cyclase from Alicyclobacillus acidocaldarius (2004), Biosci. Biotechnol. Biochem., 68, 728-738.
    View publication on PubMed
Search for more literature for 4.2.1.129

Protein Variants

Protein Variants Comment Organism
E45A production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced Alicyclobacillus acidocaldarius
E93A production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced Alicyclobacillus acidocaldarius
F434A production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures Alicyclobacillus acidocaldarius
F437A production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures Alicyclobacillus acidocaldarius
G262A the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene Alicyclobacillus acidocaldarius
P263A the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene Alicyclobacillus acidocaldarius
P263G the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene Alicyclobacillus acidocaldarius
Q262G the mutant produces hopanol as the main product instead of hop-22(29)-ene. The mutant also produces hop-21(22)ene Alicyclobacillus acidocaldarius
R127Q production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced Alicyclobacillus acidocaldarius
T41A production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced Alicyclobacillus acidocaldarius
W133A production of hop-22(29)-ene is less throughout the entire temperature range than that by the wild-type. Hop-21(22)ene is not produced Alicyclobacillus acidocaldarius
Y267A production of hop-22(29)-ene is decreased, production of hopanol is markedly increased at lower temperatures Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0162
-
 squalene pH 6.0, 45°C, wild-type enzyme Alicyclobacillus acidocaldarius
0.0167
-
 squalene pH 6.0, 45°C, mutant T41A Alicyclobacillus acidocaldarius
0.0169
-
 squalene pH 6.0, 45°C, mutant W133A Alicyclobacillus acidocaldarius
0.0189
-
 squalene pH 6.0, 45°C, mutant E93A Alicyclobacillus acidocaldarius
0.0213
-
 squalene pH 6.0, 45°C, mutant E45A Alicyclobacillus acidocaldarius
0.0255
-
 squalene pH 6.0, 45°C, mutant R127Q Alicyclobacillus acidocaldarius
0.0816
-
 squalene pH 6.0, 45°C, mutant F434A Alicyclobacillus acidocaldarius
0.0955
-
 squalene pH 6.0, 45°C, mutant F437A Alicyclobacillus acidocaldarius
0.102
-
 squalene pH 6.0, 45°C, mutant Q262A Alicyclobacillus acidocaldarius
0.156
-
 squalene pH 6.0, 45°C, mutant Q262G Alicyclobacillus acidocaldarius
0.185
-
 squalene pH 6.0, 45°C, mutant P263A Alicyclobacillus acidocaldarius
0.197
-
 squalene pH 6.0, 45°C, mutant Y267A Alicyclobacillus acidocaldarius
0.237
-
 squalene pH 6.0, 45°C, mutant P263G Alicyclobacillus acidocaldarius

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hopan-22-ol
-
 Alicyclobacillus acidocaldarius squalene + H2O
-
 ?

Synonyms

Synonyms Comment Organism
squalene-hopene cyclase ambiguous Alicyclobacillus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 50 mutant Q262G Alicyclobacillus acidocaldarius
45
-
 mutant F437A Alicyclobacillus acidocaldarius
45 55 mutant Q262A Alicyclobacillus acidocaldarius
50
-
 mutant P263G, mutant P263A, mutant E45A, mutant R127Q Alicyclobacillus acidocaldarius
50 60 mutant Y267A Alicyclobacillus acidocaldarius
55
-
 mutant E93A, mutant W133A Alicyclobacillus acidocaldarius
60
-
 wild-type, mutant T41A, mutant F434A Alicyclobacillus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 squalene pH 6.0, 45°C, mutant F434A Alicyclobacillus acidocaldarius
0.163
-
 squalene pH 6.0, 45°C, mutant T41A Alicyclobacillus acidocaldarius
0.163
-
 squalene pH 6.0, 45°C, wild-type enzyme Alicyclobacillus acidocaldarius
0.17
-
 squalene pH 6.0, 45°C, mutant E93A Alicyclobacillus acidocaldarius
0.19
-
 squalene pH 6.0, 45°C, mutant W133A Alicyclobacillus acidocaldarius
0.21
-
 squalene pH 6.0, 45°C, mutant R127Q Alicyclobacillus acidocaldarius
0.21
-
 squalene pH 6.0, 45°C, mutant Y267A Alicyclobacillus acidocaldarius
0.22
-
 squalene pH 6.0, 45°C, mutant E45A Alicyclobacillus acidocaldarius
0.49
-
 squalene pH 6.0, 45°C, mutant F437A Alicyclobacillus acidocaldarius
2.03
-
 squalene pH 6.0, 45°C, mutant Q262A Alicyclobacillus acidocaldarius
3.78
-
 squalene pH 6.0, 45°C, mutant P263A Alicyclobacillus acidocaldarius
3.86
-
 squalene pH 6.0, 45°C, mutant P263G Alicyclobacillus acidocaldarius
4.4
-
 squalene pH 6.0, 45°C, mutant Q262G Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Alicyclobacillus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.07
-
 squalene pH 6.0, 45°C, mutant Y267A Alicyclobacillus acidocaldarius
1.84
-
 squalene pH 6.0, 45°C, mutant F434A Alicyclobacillus acidocaldarius
5.13
-
 squalene pH 6.0, 45°C, mutant F437A Alicyclobacillus acidocaldarius
8.24
-
 squalene pH 6.0, 45°C, mutant R127Q Alicyclobacillus acidocaldarius
8.99
-
 squalene pH 6.0, 45°C, mutant E93A Alicyclobacillus acidocaldarius
9.8
-
 squalene pH 6.0, 45°C, mutant T41A Alicyclobacillus acidocaldarius
10.06
-
 squalene pH 6.0, 45°C, wild-type enzyme Alicyclobacillus acidocaldarius
11.2
-
 squalene pH 6.0, 45°C, mutant W133A Alicyclobacillus acidocaldarius
16.3
-
 squalene pH 6.0, 45°C, mutant P263G Alicyclobacillus acidocaldarius
19.9
-
 squalene pH 6.0, 45°C, mutant Q262A Alicyclobacillus acidocaldarius
20.4
-
 squalene pH 6.0, 45°C, mutant E45A Alicyclobacillus acidocaldarius
20.43
-
 squalene pH 6.0, 45°C, mutant P263A Alicyclobacillus acidocaldarius
28.2
-
 squalene pH 6.0, 45°C, mutant Q262G Alicyclobacillus acidocaldarius