Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol | Castellaniella defragrans | |
additional information | the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool | Castellaniella defragrans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Castellaniella defragrans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely | Castellaniella defragrans | |
MgCl2 | high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely | Castellaniella defragrans | |
additional information | not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity | Castellaniella defragrans | |
NaCl | high salt concentrations (NaCl, KCl, or MgCl2 at a concentration of 220 mM) inhibit the enzyme activity completely | Castellaniella defragrans | |
O2 | the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool | Castellaniella defragrans | |
pyridoxal 5'-phosphate | 1 mM; 35% inhibition | Castellaniella defragrans | |
S-adenosyl-L-methionine | 40 mM, 80% inhibition | Castellaniella defragrans | |
Ti(III)citrate | 1 mM, complete inhibition | Castellaniella defragrans | |
Urea | 20% activity remains at 3 M urea, and no activity is detected in 6 M urea | Castellaniella defragrans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
linalool | pH 9.0, 35°C | Castellaniella defragrans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Castellaniella defragrans |
160000 | - |
gel filtration | Castellaniella defragrans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myrcene + H2O | Castellaniella defragrans | degradation of myrcene | linalool | - |
r | |
myrcene + H2O | Castellaniella defragrans 65Phen | degradation of myrcene | linalool | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Castellaniella defragrans | E1XUJ2 | - |
- |
Castellaniella defragrans 65Phen | E1XUJ2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | precursor protein with a signal peptide for a periplasmatic location | Castellaniella defragrans |
Purification (Comment) | Organism |
---|---|
- |
Castellaniella defragrans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.55 | - |
pH 9.0, 35°C | Castellaniella defragrans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linalool | dehydration of linalool to myrcene in the absence of molecular oxygen. The aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyzes in vitro the reaction in both directions depending on the thermodynamic driving forces | Castellaniella defragrans | myrcene + H2O | myrcene is the only product detected | r | |
linalool | dehydration of linalool to myrcene in the absence of molecular oxygen. The aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol. The enzyme catalyzes in vitro the reaction in both directions depending on the thermodynamic driving forces | Castellaniella defragrans 65Phen | myrcene + H2O | myrcene is the only product detected | r | |
additional information | neither the monoterpenes alpha- and beta-ocimene nor the monoterpenoids citronellol and nerol are transformed | Castellaniella defragrans | ? | - |
? | |
additional information | neither the monoterpenes alpha- and beta-ocimene nor the monoterpenoids citronellol and nerol are transformed | Castellaniella defragrans 65Phen | ? | - |
? | |
myrcene + H2O | degradation of myrcene | Castellaniella defragrans | linalool | - |
r | |
myrcene + H2O | degradation of myrcene | Castellaniella defragrans 65Phen | linalool | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Castellaniella defragrans |
Synonyms | Comment | Organism |
---|---|---|
linalool dehydratase-isomerase | bifunctional enzyme that catalyzes the reversible dehydration and isomerization of linalool (3,7-dimethyl-1,6-octadien-3-ol) | Castellaniella defragrans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Castellaniella defragrans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | 40 | 28°C: about 60% of maximal activity, 40°C: about 80% of maximal activity | Castellaniella defragrans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
Tris-HCl buffer, sharp decrease in linalool dehydratase activity beyond pH 9.0 | Castellaniella defragrans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | contains no prosthetic group | Castellaniella defragrans |