Literature summary for 4.2.1.126 extracted from

Ruscitto, A.; Hottmann, I.; Stafford, G.P.; Schaeffer, C.; Mayer, C.; Sharma, A.
Identification of a novel N-acetylmuramic acid transporter in Tannerella forsythia (2016), J. Bacteriol., 198, 3119-3125 .

Search for more literature for 4.2.1.126

Cloned(Commentary)

Cloned (Comment)	Organism
gene murQ, DNA and amino acid sequence determination and analysis, genetic organization of the MurNAc utilization locus, the three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Complementation with the Tanf_08375 and Tanf_08380 genes together in trans, but not individually, rescues the inability of an Escherichia coli mutant deficient in the phosphotransferase (PTS) system-dependent MurNAc transporter MurP as well as that of a double mutant deficient in MurP and components of the PTS system to grow on MurNAc. In addition, complementation with this two-gene construct in Escherichia coli causes depletion of MurNAc in the medium. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Tannerella forsythia

Cloned (Comment)

Organism

gene murQ, DNA and amino acid sequence determination and analysis, genetic organization of the MurNAc utilization locus, the three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Complementation with the Tanf_08375 and Tanf_08380 genes together in trans, but not individually, rescues the inability of an Escherichia coli mutant deficient in the phosphotransferase (PTS) system-dependent MurNAc transporter MurP as well as that of a double mutant deficient in MurP and components of the PTS system to grow on MurNAc. In addition, complementation with this two-gene construct in Escherichia coli causes depletion of MurNAc in the medium. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Tannerella forsythia

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	Tannerella forsythia	-	N-acetylmuramate 6-phosphate + H2O	-	r
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338	-	N-acetylmuramate 6-phosphate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Tannerella forsythia	G8UQI0	i.e. Bacteroides forsythus	-
Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338	G8UQI0	i.e. Bacteroides forsythus	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Tannerella forsythia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	-	Tannerella forsythia	N-acetylmuramate 6-phosphate + H2O	-	r
(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	-	Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338	N-acetylmuramate 6-phosphate + H2O	-	r
additional information	the etherase activity is assessed by utilizing N-acetylmuramate 6-phosphate as the substrate in a Morgan Elson reaction. This etherase-catalyzed reaction generates a chromogenic intermediate that can be detected by reacting with Ehrlich's reagent dimethylaminobenzaldehyde to yield a purple product. Also, radioactive assay with 32P-radiolabeled substrates N-acetyl-D-glucosamine 6-phosphate and N-acetylmuramate 6-phosphate	Tannerella forsythia	?	-	?
additional information	the etherase activity is assessed by utilizing N-acetylmuramate 6-phosphate as the substrate in a Morgan Elson reaction. This etherase-catalyzed reaction generates a chromogenic intermediate that can be detected by reacting with Ehrlich's reagent dimethylaminobenzaldehyde to yield a purple product. Also, radioactive assay with 32P-radiolabeled substrates N-acetyl-D-glucosamine 6-phosphate and N-acetylmuramate 6-phosphate	Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338	?	-	?
N-acetylmuramate 6-phosphate + H2O	-	Tannerella forsythia	(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	-	r
N-acetylmuramate 6-phosphate + H2O	-	Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338	(R)-lactate + N-acetyl-D-glucosamine 6-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
MurQ	-	Tannerella forsythia
MurQ etherase	-	Tannerella forsythia
Tanf_08385	-	Tannerella forsythia
TfMurQ	-	Tannerella forsythia

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	45	assay at	Tannerella forsythia

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	7.6	assay at	Tannerella forsythia

General Information

General Information	Comment	Organism
metabolism	MurNAc utilization locus and MurNAc utilization pathway in Tannerella forsythia, overview. The three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Enzymes MurT-MurK function as a PTS-independent MurNAc transporter	Tannerella forsythia
physiological function	the enzyme is involved in the metabolic conversion of MurNAc-6-phosphate to GlcNAc-6-phosphate	Tannerella forsythia