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Literature summary for 4.2.1.126 extracted from
- Scission of the lactyl ether bond of N-acetylmuramic acid by Escherichia coli etherase (2005), J. Biol. Chem., 280, 30100-30106.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| MurQ-His fusion protein is expressed in Escherichia coli BL21 cells | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap chelating HP column chromatography, gel filtration | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, imidazole buffer (pH 8.0) by adding 20% glycerol and 1 mM dithiothreitol, at least several weeks, activity is retained | Escherichia coli |
| 4°C, purified etherase is only active for a few days | Escherichia coli |
| temperature, storage medium, duration, loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | N-acetylmuramate is not a substrate nor are anhydro-N-acetylmuramate or muramic acid | Escherichia coli | ? | - |
? |  |
| N-acetylmuramate 6-phosphate + H2O | - |
Escherichia coli | D-lactate + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurNAc etherase | - |
Escherichia coli |
| MurQ | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a murQ deletion mutant cannot grow on N-acetylmuramic acid as the sole source of carbon | Escherichia coli |
| physiological function | MurQ is required for growth on N-acetylmuramic acid | Escherichia coli |
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Release 2023.1 (January 2023)
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