Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D115N | the mutant exhibits a 7fold reduction in the value of kcat | Escherichia coli |
E114Q | the mutant shows a dramatic reduction in the value of kcat (2000fold) and a modest decrease in the value of KM (4fold) | Escherichia coli |
E83A | the mutant is essentially inactive, the mutant shows a very low, but measurable, level of activity with a kcat value that wis 10000fold lower than that of the wild type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 5 mM EDTA results in only weak inhibition (25% reduction in rate) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
N-acetylmuramate 6-phosphate | mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
0.83 | - |
N-acetylmuramate 6-phosphate | mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
1.2 | - |
N-acetylmuramate 6-phosphate | wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating Sepharose column chromatography, gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-3-deoxy-N-acetylglucosamine 6-phosphate | slow conversion, 2% activity compared to N-acetylmuramate 6-phosphate | Escherichia coli | (2E)-2-(acetylamino)-2,3-dideoxy-6-O-phosphono-D-erythro-hex-2-enose + HCl | - |
? | |
N-acetylmuramate 6-phosphate + H2O | - |
Escherichia coli | (R)-lactate + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MurNAc 6-phosphate etherase | - |
Escherichia coli |
MurNAc 6-phosphate etherase lyase | - |
Escherichia coli |
MurNAc 6-phosphate hydrolase | - |
Escherichia coli |
MurQ | - |
Escherichia coli |
N-acetylmuramic acid 6-phosphate hydrolase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
N-acetylmuramate 6-phosphate | mutant enzyme E83A, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
0.0028 | - |
N-acetylmuramate 6-phosphate | mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
0.79 | - |
N-acetylmuramate 6-phosphate | mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
5.7 | - |
N-acetylmuramate 6-phosphate | wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0088 | - |
N-acetylmuramate 6-phosphate | mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
0.95 | - |
N-acetylmuramate 6-phosphate | mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli | |
4.8 | - |
N-acetylmuramate 6-phosphate | wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) | Escherichia coli |