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Literature summary for 4.2.1.126 extracted from

  • Hadi, T.; Dahl, U.; Mayer, C.; Tanner, M.E.
    Mechanistic studies on N-acetylmuramic acid 6-phosphate hydrolase (MurQ): an etherase involved in peptidoglycan recycling (2008), Biochemistry, 47, 11547-11558.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D115N the mutant exhibits a 7fold reduction in the value of kcat Escherichia coli
E114Q the mutant shows a dramatic reduction in the value of kcat (2000fold) and a modest decrease in the value of KM (4fold) Escherichia coli
E83A the mutant is essentially inactive, the mutant shows a very low, but measurable, level of activity with a kcat value that wis 10000fold lower than that of the wild type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM EDTA results in only weak inhibition (25% reduction in rate) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
N-acetylmuramate 6-phosphate mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
0.83
-
N-acetylmuramate 6-phosphate mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
1.2
-
N-acetylmuramate 6-phosphate wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating Sepharose column chromatography, gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-3-deoxy-N-acetylglucosamine 6-phosphate slow conversion, 2% activity compared to N-acetylmuramate 6-phosphate Escherichia coli (2E)-2-(acetylamino)-2,3-dideoxy-6-O-phosphono-D-erythro-hex-2-enose + HCl
-
?
N-acetylmuramate 6-phosphate + H2O
-
Escherichia coli (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
MurNAc 6-phosphate etherase
-
Escherichia coli
MurNAc 6-phosphate etherase lyase
-
Escherichia coli
MurNAc 6-phosphate hydrolase
-
Escherichia coli
MurQ
-
Escherichia coli
N-acetylmuramic acid 6-phosphate hydrolase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
N-acetylmuramate 6-phosphate mutant enzyme E83A, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
0.0028
-
N-acetylmuramate 6-phosphate mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
0.79
-
N-acetylmuramate 6-phosphate mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
5.7
-
N-acetylmuramate 6-phosphate wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0088
-
N-acetylmuramate 6-phosphate mutant enzyme E114Q, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
0.95
-
N-acetylmuramate 6-phosphate mutant enzyme D115N, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli
4.8
-
N-acetylmuramate 6-phosphate wild type enzyme, at 30°C in 60 mM Trien-HCl buffer (pH 8.0) Escherichia coli