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Literature summary for 4.2.1.122 extracted from

  • Blei, F.; Baldeweg, F.; Fricke, J.; Hoffmeister, D.
    Biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions (2018), Chemistry, 24, 10028-10031 .
    View publication on PubMed
Search for more literature for 4.2.1.122

Application

Application Comment Organism
synthesis the enzyme participates in psilocybin formation from 4-hydroxyindole and L-serine, which are less cost-intensive substrates, compared to the previous method. The pharmaceutical interest in this psychotropic natural product as a future medication to treat depression and anxiety is strongly reemerging. Enzymatic production of 7-phosphoryloxytryptamine (isonorbaeocystin), a non-natural congener of the Psilocybe alkaloid norbaeocystin (4-phosphoryloxytryptamine), and of serotonin (5-hydroxytryptamine) by means of the same in vitro approach Psilocybe cubensis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His6-tagged TrpB in Escherichia coli strain KRX Psilocybe cubensis

Protein Variants

Protein Variants Comment Organism
additional information biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions, in vitro reconstituted indole alkaloid synthesis pathway including enzyme PsiD, PsiK and PsiM, and ATP and S-adenosyl-L-methionine, method, overview. Assays run only with TrpB and PsiD result in identical chromatograms Psilocybe cubensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Psilocybe cubensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + indole Psilocybe cubensis
-
 L-tryptophan + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Psilocybe cubensis A0A2U8JPY3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + 4-hydroxyindole
-
 Psilocybe cubensis 4-hydroxy-L-tryptophan + H2O
-
 ?
L-serine + 5-hydroxyindole
-
 Psilocybe cubensis 5-hydroxy-L-tryptophan + H2O
-
 ?
L-serine + 7-hydroxyindole
-
 Psilocybe cubensis 7-hydroxy-L-tryptophan + H2O
-
 ?
L-serine + indole
-
 Psilocybe cubensis L-tryptophan + H2O
-
 ?
L-serine + indole L-serine is the preferred substrate Psilocybe cubensis L-tryptophan + H2O
-
 ?
additional information enzymatic production of psilocybin formation from 4-hydroxyindole and L-serine and of 7-phosphoryloxytryptamine (isonorbaeocystin), a non-natural congener of the Psilocybe alkaloid norbaeocystin (4-phosphoryloxytryptamine), and of serotonin (5-hydroxytryptamine) by means of the same in vitro approach, overview Psilocybe cubensis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 75500, about, TrpB monomer, sequence calculation Psilocybe cubensis

Synonyms

Synonyms Comment Organism
TrpBbeta
-
 Psilocybe cubensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Psilocybe cubensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Psilocybe cubensis

pI Value

Organism Comment pI Value Maximum pI Value
Psilocybe cubensis TrpB monomer, sequence calculation
-
 5.9

General Information

General Information Comment Organism
physiological function the enzyme is part of the tryptophan synthase complex. Indole formation is catalyzed by the alpha-subunit (TrpBalpha), L-tryptophan production is catalyzed by the beta-subunit (TrpBbeta) Psilocybe cubensis