Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
indole | Km below 0.00077 mM, at 80°C, pH not specified in the publication | Thermotoga maritima | |
50.2 | - |
L-serine | at 80°C, pH not specified in the publication | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46400 | - |
2 * 46400, calculated from amino acid sequence | Thermotoga maritima |
61700 | - |
gel filtration | Thermotoga maritima |
92770 | - |
calculated from amino acid sequence | Thermotoga maritima |
98000 | - |
sedimentation equilibrium analysis | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Mono Q column chromatography and Superdex 75 gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + indole | - |
Thermotoga maritima | L-tryptophan + H2O | - |
? | |
additional information | TrpB2 does not bind to TrpA but is catalytically highly active, has an extremely low Km value for indole | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 46400, calculated from amino acid sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
TrpB2 | - |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
L-serine | at 80°C, pH not specified in the publication | Thermotoga maritima | |
0.46 | - |
indole | at 80°C, pH not specified in the publication | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | TrpB2 acts as an indole rescue protein, which prevents the escape of this costly hydrophobic metabolite from the cell at the high growth temperatures of hyperthermophiles | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
indole | KCAT_KM below 0.0006 mM, at 80°C, pH not specified in the publication | Thermotoga maritima | |
8700 | - |
L-serine | at 80°C, pH not specified in the publication | Thermotoga maritima |