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Literature summary for 4.2.1.122 extracted from

  • Hettwer, S.; Sterner, R.
    A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role (2002), J. Biol. Chem., 277, 8194-8201.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00077
-
indole Km below 0.00077 mM, at 80°C, pH not specified in the publication Thermotoga maritima
50.2
-
L-serine at 80°C, pH not specified in the publication Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46400
-
2 * 46400, calculated from amino acid sequence Thermotoga maritima
61700
-
gel filtration Thermotoga maritima
92770
-
calculated from amino acid sequence Thermotoga maritima
98000
-
sedimentation equilibrium analysis Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mono Q column chromatography and Superdex 75 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + indole
-
Thermotoga maritima L-tryptophan + H2O
-
?
additional information TrpB2 does not bind to TrpA but is catalytically highly active, has an extremely low Km value for indole Thermotoga maritima ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 46400, calculated from amino acid sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
TrpB2
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
L-serine at 80°C, pH not specified in the publication Thermotoga maritima
0.46
-
indole at 80°C, pH not specified in the publication Thermotoga maritima

General Information

General Information Comment Organism
physiological function TrpB2 acts as an indole rescue protein, which prevents the escape of this costly hydrophobic metabolite from the cell at the high growth temperatures of hyperthermophiles Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0006
-
indole KCAT_KM below 0.0006 mM, at 80°C, pH not specified in the publication Thermotoga maritima
8700
-
L-serine at 80°C, pH not specified in the publication Thermotoga maritima