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Literature summary for 4.2.1.118 extracted from

  • Fox, D.T.; Hotta, K.; Kim, C.Y.; Koppisch, A.T.
    The missing link in petrobactin biosynthesis: asbF encodes a (-)-3-dehydroshikimate dehydratase (2008), Biochemistry, 47, 12251-12253.
    View publication on PubMed
Search for more literature for 4.2.1.118

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus thuringiensis

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, complete loss of activity. More than 95% of activity can be restored in presence of Mg2+ Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 (-)-3-dehydroshikimate 37°C Bacillus thuringiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Bacillus thuringiensis
Mg2+ presence of Mg2+ restores activity after incubation with EDTA Bacillus thuringiensis
Mn2+
-
 Bacillus thuringiensis
additional information a divalent metal ion is absolutely required Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis
-
-
-
Bacillus thuringiensis 97-27
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
complete loss of activity in presence of EDTA. More than 95% of activity can be restored in presence of Mg2+ Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-3-dehydroshikimate
-
 Bacillus thuringiensis 3,4-dihydroxybenzoate + H2O
-
 ?
(-)-3-dehydroshikimate
-
 Bacillus thuringiensis 97-27 3,4-dihydroxybenzoate + H2O
-
 ?
additional information enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 Bacillus thuringiensis ?
-
 ?
additional information enzyme displays to ionizable groups with pKa1 7.9, and pKa2 9.3 Bacillus thuringiensis 97-27 ?
-
 ?

Synonyms

Synonyms Comment Organism
(-)-3-dehydroshikimate dehydratase
-
 Bacillus thuringiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
46
-
 half-life 10 min Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 (-)-3-dehydroshikimate 37°C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.8
-
 Bacillus thuringiensis

pH Range

pH Minimum pH Maximum Comment Organism
7.4
-
 less than 15% residual activity Bacillus thuringiensis