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Literature summary for 4.2.1.115 extracted from
- Mechanistic studies on PseB of pseudaminic acid biosynthesis: a UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase (2008), Bioorg. Chem., 36, 312-320.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | PseB is a potential target for the development of antibiotics | Campylobacter jejuni |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pseB, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D126N | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to D126N the tightly bound NADPH is immediately oxidized | Campylobacter jejuni |
| K127A | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to K127A the tightly bound NADPH is immediately oxidized | Campylobacter jejuni |
| Y135F | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme, slow oxidation of NADPH upon addition of UDP-4-keto-6-deoxy-GlcNAc to Y135F | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-GlcNAc | Campylobacter jejuni | the enzyme is involved in the pseudaminic acid biosynthesis, it is responsible for the biosynthesis of 6-deoxyhexose | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | - |
gene pseB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | Asp126, Lys127, and Tyr135 are the active site residues | Campylobacter jejuni |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Campylobacter jejuni |
| 0.000037 | - |
wild-type enzyme activity with UDP-6-deoxy-6-fluoro-GlcNAc for elimination of fluoride | Campylobacter jejuni |
| 0.23 | - |
wild-type enzyme activity with UDP-GlcNAc conversion to UDP-4-keto-6-deoxy-L-IdoNAc | Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-6-deoxy-6-fluoro-GlcNAc | elimination of fluoride from the substrate by the wild-type PseB, no activity by mutant enzymes K127A, D126N, and Y135F | Campylobacter jejuni | UDP-GlcNAc + HF | - |
? | |
| UDP-GlcNAc | the enzyme is involved in the pseudaminic acid biosynthesis, it is responsible for the biosynthesis of 6-deoxyhexose | Campylobacter jejuni | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? | |
| UDP-GlcNAc | the C-5'' epimerization of UDP-4-keto-6-deoxy-L-IdoNAc to UDP-4-keto-6-deoxy-GlcNAc is PseB-catalyzed, and is about 50fold lower than the dehydratase activity | Campylobacter jejuni | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a member of the short chain dehydrogenase/reductase, SDR, family | Campylobacter jejuni |
| PseB | - |
Campylobacter jejuni |
| UDP-GlcNAc 5-inverting 4,6-dehydratase | - |
Campylobacter jejuni |
| UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase | - |
Campylobacter jejuni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 30 | assay at | Campylobacter jejuni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Campylobacter jejuni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | dependent on, tightly bound | Campylobacter jejuni | |
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