Protein Variants | Comment | Organism |
---|---|---|
K163R | no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(S)-phenylglycine with solvent hydrogen | Amycolatopsis sp. |
K163S | no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(S)-phenylglycine with solvent hydrogen | Amycolatopsis sp. |
K263R | no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(R)-phenylglycine with solvent hydrogen | Amycolatopsis sp. |
K263S | no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(R)-phenylglycine with solvent hydrogen | Amycolatopsis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | - |
Amycolatopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | Amycolatopsis sp. | reaction in menaquinone biosynthetic pathway | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | - |
Amycolatopsis sp. | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? | |
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | reaction in menaquinone biosynthetic pathway | Amycolatopsis sp. | 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O | - |
? | |
additional information | the enzyme also shows N-acylamino acid racemase activity, with the optimal substrate being the enantiomers of N-acetyl methionine | Amycolatopsis sp. | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate | - |
Amycolatopsis sp. |