The anhydrofructose pathway and its possible role in stress response and signaling

Yu, S.; Fiskesund, R.; Biochim. Biophys. Acta 1760, 1314-1322 (2006)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

EDTA

50 mM, 84% inhibition

Anthracobia melaloma

Metals/Ions

Commentary

Organism

Structure

CaCl2

requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity

Anthracobia melaloma

MgCl2

requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity

Anthracobia melaloma

NaCl

0.1 mM, around 2fold increase in activity

Anthracobia melaloma

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Anthracobia melaloma

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Plicaria carbonaria

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Plicaria leiocarpa

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

additional information

the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone

667822

Anthracobia melaloma

?

-

-

-

?

Inhibitors

Commentary

Organism

Structure

EDTA

50 mM, 84% inhibition

Anthracobia melaloma

Metals/Ions

Commentary

Organism

Structure

CaCl2

requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity

Anthracobia melaloma

MgCl2

requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity

Anthracobia melaloma

NaCl

0.1 mM, around 2fold increase in activity

Anthracobia melaloma

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Anthracobia melaloma

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Plicaria carbonaria

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

1,5-anhydro-D-fructose

i.e. 1,5-anhydro-D-arabino-hex-2-ulose

667822

Plicaria leiocarpa

1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O

i.e.ascopyrone M

-

-

ir

additional information

the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone

667822

Anthracobia melaloma

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

703073

Lundt

1,5-Anhydro-D-fructose: biocat ...

Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa, Peziza petersii

Carbohydr. Res.

345

181-190

2010

-

-

-

-

-

-

1

-

-

3

-

-

-

4

-

-

-

-

-

-

-

-

8

-

4

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

3

-

-

-

-

-

-

-

-

-

-

8

-

-

-

-

-

1

1

-

-

-

-

-

-

-

-

667822

Yu

The anhydrofructose pathway an ...

Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa

Biochim. Biophys. Acta

1760

1314-1322

2006

-

-

-

-

-

-

1

-

-

3

2

-

-

3

-

-

-

-

-

-

-

-

4

1

6

-

-

-

-

1

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

3

2

-

-

-

-

-

-

-

-

-

4

1

-

-

-

-

1

1

-

-

-

-

-

-

-

-

650418

Yu

Enzymatic description of the a ...

Anthracobia melaloma

Biochim. Biophys. Acta

1672

120-129

2004

-

-

-

-

-

-

-

-

-

-

2

1

-

1

-

-

1

-

-

-

1

-

2

1

6

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

2

1

-

-

-

1

-

-

1

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-