BRENDA - Enzyme Database
show all sequences of 4.2.1.111

The anhydrofructose pathway and its possible role in stress response and signaling

Yu, S.; Fiskesund, R.; Biochim. Biophys. Acta 1760, 1314-1322 (2006)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
50 mM, 84% inhibition
Anthracobia melaloma
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CaCl2
requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity
Anthracobia melaloma
MgCl2
requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity
Anthracobia melaloma
NaCl
0.1 mM, around 2fold increase in activity
Anthracobia melaloma
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98000
-
2 * 98000, SDS-PAGE
Anthracobia melaloma
230000
-
gel filtration
Anthracobia melaloma
Organism
Organism
UniProt
Commentary
Textmining
Anthracobia melaloma
-
-
-
Plicaria carbonaria
-
-
-
Plicaria leiocarpa
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Plicaria carbonaria
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Plicaria leiocarpa
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
additional information
the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone
667822
Anthracobia melaloma
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 98000, SDS-PAGE
Anthracobia melaloma
Synonyms
Synonyms
Commentary
Organism
AF dehydratase
-
Anthracobia melaloma
AF dehydratase
-
Plicaria carbonaria
AF dehydratase
-
Plicaria leiocarpa
AFDH
-
Anthracobia melaloma
AFDH
-
Plicaria carbonaria
AFDH
-
Plicaria leiocarpa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
optimal pH-range is 5.9-7.0
Anthracobia melaloma
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.9
7
optimal pH-range
Anthracobia melaloma
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
50 mM, 84% inhibition
Anthracobia melaloma
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CaCl2
requirement of alkaline earth metal ion for activity. 10 mM, around 2fold increase in activity
Anthracobia melaloma
MgCl2
requirement of alkaline earth metal ion for activity. 25 mM, around 2fold increase in activity
Anthracobia melaloma
NaCl
0.1 mM, around 2fold increase in activity
Anthracobia melaloma
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98000
-
2 * 98000, SDS-PAGE
Anthracobia melaloma
230000
-
gel filtration
Anthracobia melaloma
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Plicaria carbonaria
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
667822
Plicaria leiocarpa
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
additional information
the enzyme is inactive towards: glucosone, glucose, galactose, mannose, fructose, ribose and gamma-gluconolactone
667822
Anthracobia melaloma
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 98000, SDS-PAGE
Anthracobia melaloma
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
optimal pH-range is 5.9-7.0
Anthracobia melaloma
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.9
7
optimal pH-range
Anthracobia melaloma
Other publictions for EC 4.2.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703073
Lundt
1,5-Anhydro-D-fructose: biocat ...
Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa, Peziza petersii
Carbohydr. Res.
345
181-190
2010
-
-
-
-
-
-
1
-
-
3
-
-
-
4
-
-
-
-
-
-
-
-
8
-
4
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
667822
Yu
The anhydrofructose pathway an ...
Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa
Biochim. Biophys. Acta
1760
1314-1322
2006
-
-
-
-
-
-
1
-
-
3
2
-
-
3
-
-
-
-
-
-
-
-
4
1
6
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
2
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
650418
Yu
Enzymatic description of the a ...
Anthracobia melaloma
Biochim. Biophys. Acta
1672
120-129
2004
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-