BRENDA - Enzyme Database
show all sequences of 4.2.1.111

Enzymatic description of the anhydrofructose pathway of glycogen degradation; I. Identification and purification of anhydrofructose dehydratase, ascopyrone tautomerase and alpha-1,4-glucan lyase in the fungus Anthracobia melaloma

Yu, S.; Refdahl, C.; Lundt, I.; Biochim. Biophys. Acta 1672, 120-129 (2004)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98000
-
x * 98000, SDS-PAGE
Anthracobia melaloma
140000
-
gel filtration
Anthracobia melaloma
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1,5-anhydro-D-fructose
Anthracobia melaloma
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Anthracobia melaloma
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Anthracobia melaloma
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1870
-
-
Anthracobia melaloma
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose
650418
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
650418
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
Subunits
Subunits
Commentary
Organism
?
x * 98000, SDS-PAGE
Anthracobia melaloma
Synonyms
Synonyms
Commentary
Organism
1,5-anhydro-D-arabino-hex-2-ulose dehydratase
-
Anthracobia melaloma
1,5-anhydro-D-fructose 4-dehydratase
-
Anthracobia melaloma
1,5-anhydro-D-fructose hydro-lyase
-
Anthracobia melaloma
1,5-anhydro-D-fructose hydrolyase
-
Anthracobia melaloma
AF dehydratase
-
Anthracobia melaloma
AFDH
-
Anthracobia melaloma
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
98000
-
x * 98000, SDS-PAGE
Anthracobia melaloma
140000
-
gel filtration
Anthracobia melaloma
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1,5-anhydro-D-fructose
Anthracobia melaloma
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
-
Anthracobia melaloma
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1870
-
-
Anthracobia melaloma
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose. This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose
650418
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
1,5-anhydro-D-fructose
i.e. 1,5-anhydro-D-arabino-hex-2-ulose
650418
Anthracobia melaloma
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose + H2O
i.e.ascopyrone M
-
-
ir
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 98000, SDS-PAGE
Anthracobia melaloma
Other publictions for EC 4.2.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
703073
Lundt
1,5-Anhydro-D-fructose: biocat ...
Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa, Peziza petersii
Carbohydr. Res.
345
181-190
2010
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667822
Yu
The anhydrofructose pathway an ...
Anthracobia melaloma, Plicaria carbonaria, Plicaria leiocarpa
Biochim. Biophys. Acta
1760
1314-1322
2006
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4
1
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1
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650418
Yu
Enzymatic description of the a ...
Anthracobia melaloma
Biochim. Biophys. Acta
1672
120-129
2004
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6
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