Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.110 extracted from

  • Koths, K.; Halenbeck, R.; Moreland, M.
    Synthesis of the antibiotic cortalcerone from D-glucose using pyranose 2-oxidase and a novel fungal enzyme, aldos-2-ulose dehydratase (1992), Carbohydr. Res., 232, 59-75.
    View publication on PubMed
Search for more literature for 4.2.1.110

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 2 * 95000, SDS-PAGE Polyporus obtusus
200000
-
 gel filtration Polyporus obtusus

Organism

Organism UniProt Comment Textmining
Polyporus obtusus
-
-
-
Polyporus obtusus AU124PD
-
-
-

Purification (Commentary)

Purification (Comment) Organism
precipitation with 20% (w/v) PEG 4000, DEAE-Sepharose column chromatography, and carboxymethyl-Sepharose column chromatography Polyporus obtusus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Polyporus obtusus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 cleared lysate, at pH 6.0 and 25°C Polyporus obtusus
9
-
 after 450fold purification, at pH 6.0 and 25°C Polyporus obtusus

Storage Stability

Storage Stability Organism
4°C, purified enzyme in 0.1 M sodium phosphate buffer (pH 7.0), several months, the enzyme remains stable Polyporus obtusus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosone i.e. D-arabino-hexos-2-ulose. The enzyme dehydrates D-arabino-hexos-2-ulose, generating a double bond between C-3 and C-4. This unsaturated compound is proposed to be the steady-state intermediate characterized by the spectral peak at 265 nm. This intermediate is chemically unstable and rearranges from its strained 1,5 ring form to a more stable 2,6 pyranose structure. Then the enzyme acts a second time, dehydrating this pyranose structure to form cortalcerone Polyporus obtusus cortalcerone + H2O i.e. 5,6-dihydro-6-hydroxy-5-oxo-2H-pyran-6-carboxaldehyde ?
D-glucosone i.e. D-arabino-hexos-2-ulose. The enzyme dehydrates D-arabino-hexos-2-ulose, generating a double bond between C-3 and C-4. This unsaturated compound is proposed to be the steady-state intermediate characterized by the spectral peak at 265 nm. This intermediate is chemically unstable and rearranges from its strained 1,5 ring form to a more stable 2,6 pyranose structure. Then the enzyme acts a second time, dehydrating this pyranose structure to form cortalcerone Polyporus obtusus AU124PD cortalcerone + H2O i.e. 5,6-dihydro-6-hydroxy-5-oxo-2H-pyran-6-carboxaldehyde ?
D-xylosone i.e. D-threo-pentos-2-ulose Polyporus obtusus ? the product absorbs at 260 nm but no conversion into a 230 nm-absorbing product occurs. Because of its strained nature, the intermediate still rearranges, losing UV absorbance, but since it lacks the 6th carbon, it cannot form a 2,6-pyranose ring to generate a second substrate for the enzyme ?
D-xylosone i.e. D-threo-pentos-2-ulose Polyporus obtusus AU124PD ? the product absorbs at 260 nm but no conversion into a 230 nm-absorbing product occurs. Because of its strained nature, the intermediate still rearranges, losing UV absorbance, but since it lacks the 6th carbon, it cannot form a 2,6-pyranose ring to generate a second substrate for the enzyme ?
additional information no activity with fructose Polyporus obtusus ?
-
 ?
additional information no activity with fructose Polyporus obtusus AU124PD ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 95000, SDS-PAGE Polyporus obtusus

Synonyms

Synonyms Comment Organism
aldosulose-utilizing enzyme
-
 Polyporus obtusus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Polyporus obtusus