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Literature summary for 4.2.1.11 extracted from
- Enolase from Candida albicans--purification and characterization (2000), Comp. Biochem. Physiol. B, 126, 109-120.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | noncompetitive in the presence of phosphate, competitive in the absence of phosphate | Candida albicans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.38 | - |
2-phospho-D-glycerate | pH 6.8, 30ºC | Candida albicans | |
| 0.95 | - |
phosphoenolpyruvate | pH 6.8, 30ºC | Candida albicans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, can be replaced by Mn2+ or Zn2+, but lower activity is observed | Candida albicans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, using ammonium sulfate precipitation and DEAE-Sephadex column chromatography | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 6.8, 30ºC | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Candida albicans | phosphoenolpyruvate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Candida albicans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enolase | - |
Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
heat shock protein, 50% of the activity retained after 1 h of incubation, 30% of the activity retained after 2 h of incubation | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Candida albicans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | steep decrease in activity below pH 6.5, 50% of the maximal activity retained above pH 7.0 | Candida albicans |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
F- | pH 6.8, 30ºC, in the absence of phosphate | Candida albicans | |
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