Any feedback?
Literature summary for 4.2.1.11 extracted from
- Octameric enolase from the hyperthermophilic bacterium Thermotoga maritima: purification, characterization, and image processing (1995), Protein Sci., 4, 228-236.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | in absence of phosphate noncompetitive inhibition up to 10 mM F-, competitive inhibition in presence of 0.5 mM phosphate | Thermotoga maritima |  |
| phosphate | - |
Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | temperature-dependence of Km-values | Thermotoga maritima | |
| 0.07 | - |
2-phospho-D-glycerate | at 75°C | Thermotoga maritima | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Km: 0.03 mM | Thermotoga maritima | |
| Mg2+ | absolute requirement for divalent metal ions, Mg2+ is most effective | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
8 * 48000, a tetramer of dimers, SDS-PAGE | Thermotoga maritima |
| 345000 | - |
gel filtration, high-speed equilibrium sedimentation | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Thermotoga maritima | phosphoenolpyruvate | - |
? | |
| 2-phospho-D-glycerate | - |
Thermotoga maritima | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 48000, a tetramer of dimers, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
at 70°C | Thermotoga maritima |
| 8 | - |
20°C | Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
