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Literature summary for 4.2.1.11 extracted from
- Purification, characterization, and distribution of enolase isozymes in chicken (1985), J. Biochem., 98, 1527-1534.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
2-phospho-D-glycerate | brain enolase I, brain enolase II, brain enolase III | Gallus gallus |  |
| 0.038 | - |
2-phospho-D-glycerate | muscle enolase | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE | Gallus gallus |
| 51000 | - |
alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE | Gallus gallus |
| 51500 | - |
beta,beta, 2 * 51500, muscle enolase, SDS-PAGE | Gallus gallus |
| 52000 | - |
alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE | Gallus gallus |
| 52000 | - |
gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE | Gallus gallus |
| 100000 | - |
gel filtration | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | the brain alpha,gamma and gamma,gamma forms are not detectable in the early embryonic stage and increase gradually during the development of the brain, whereas the alpha,alpha form exists at an almost constant level during development | Gallus gallus | - |
| brain | enolase I (alpha,alpha-enolase), enolase II (alpha,gamma-enolase), enolase III (gamma,gamma-enolase) | Gallus gallus | - |
| skeletal muscle | contains a single enzyme form | Gallus gallus | - |
| skeletal muscle | beta,beta-enolase, complete switching from alpha,alpha enolase to beta,beta enolase during the period around hatching | Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 79.8 | - |
brain enolase III | Gallus gallus |
| 82.8 | - |
brain enolase I | Gallus gallus |
| 92.3 | - |
muscle enolase | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Gallus gallus | phosphoenolpyruvate | - |
? | |
| 2-phospho-D-glycerate | - |
Gallus gallus | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha,gamma, 1 * 51000 + 1 * 52000, brain enolase II, SDS-PAGE | Gallus gallus |
| dimer | gamma,gamma, 2 * 52000, brain enolase III, SDS-PAGE | Gallus gallus |
| dimer | alpha,alpha, 2 * 51000, brain enolase I, SDS-PAGE | Gallus gallus |
| dimer | beta,beta, 2 * 51500, muscle enolase, SDS-PAGE | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
60 min, in presence of Mg2+, enolase II retains more than 40% of its activity, brain enolase I and II lose 80% of their activities. In absence of Mg2+ all enolases quickly lose activity | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
brain enolase I, brain enolase III | Gallus gallus |
| 6.8 | - |
muscle enolase | Gallus gallus |
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