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Literature summary for 4.2.1.11 extracted from

  • Suzuki, F.; Umeda, Y.; Kato, K.
    Rat brain enolase isozymes. Purification of three forms of enolase (1980), J. Biochem., 87, 1587-1594.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
2-phospho-D-glycerate enolase III Rattus norvegicus
0.045
-
2-phospho-D-glycerate enolase II Rattus norvegicus
0.057
-
2-phospho-D-glycerate enolase I Rattus norvegicus
0.14
-
phosphoenolpyruvate enolase III Rattus norvegicus
0.15
-
phosphoenolpyruvate enolase I Rattus norvegicus
0.19
-
phosphoenolpyruvate enolase II Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus
46000
-
2 * 46000, enolase III, SDS-PAGE Rattus norvegicus
49000
-
2 * 49000, enolase I, SDS-PAGE Rattus norvegicus
49000
-
1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
3 forms of the enzyme: enolase I, enolase II and enolase III
-

Purification (Commentary)

Purification (Comment) Organism
enolase 1, enolase II, enolase III Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain enolase I (alpha,alpha-enolase), enolase II (alpha,gamma-enolase), enolase III (gamma,gamma-enolase) Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
51
-
enolase III Rattus norvegicus
68
-
enolase II Rattus norvegicus
87
-
enolase I Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Rattus norvegicus phosphoenolpyruvate
-
?
2-phospho-D-glycerate
-
Rattus norvegicus phosphoenolpyruvate + H2O
-
?
phosphoenolpyruvate
-
Rattus norvegicus 2-phospho-D-glycerate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 49000, enolase I, SDS-PAGE Rattus norvegicus
dimer 1 * 46000 + 1 * 49000, enolase II, SDS-PAGE Rattus norvegicus
dimer 2 * 46000, enolase III, SDS-PAGE Rattus norvegicus