Protein Variants | Comment | Organism |
---|---|---|
S39A | mutant of isoenzyme 1, relative maximal velocity of 0.01% and an activation constant for Mg2+ ca. 10fold higher, compared with the native enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphonoacetohydroxamate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
2-phospho-D-glycerate | in presence of 1 mM Mg2+, native enzyme | Saccharomyces cerevisiae | |
0.131 | - |
2-phospho-D-glycerate | in presence of 1 mM Mg2+, mutant enzyme S39A | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
mutant S39A of isoenzyme 1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate | - |
? | |
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | 0.019 | 2-phospho-D-glycerate | mutant enzyme S39A, pH 7.8, 21-23°C | Saccharomyces cerevisiae | |
230 | - |
2-phospho-D-glycerate | native enolase, pH 7.8, 21-23°C | Saccharomyces cerevisiae |