Literature summary for 4.2.1.109 extracted from

Kang, W.; Yang, J.K.
Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein (2012), Acta Crystallogr. Sect. F, 68, 1518-1520.

Search for more literature for 4.2.1.109

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant overexpression of an His-tagged enzyme fragment comprising the residues 20-242 in Escherichia coli strain Rosetta 2(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme fragment of residues 20-242, hanging drop vapour diffusion method, 0.001 ml of protein solution, containing 20 mM Tris-HCl, 5% v/v glycerol, 0.1 mM TCEP, 100 mM sodium chloride, pH 8.0, is mixed with 0.001 ml of reservoir solution, containing 0.04 M citric acid, 0.06 M Bis-Tris propane, 5% v/v glycerol, 18-21% w/v PEG 3350, and equilibrated against 0.2 ml of reservoir solution, 22°C, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 2.40 A resolution	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme fragment of residues 20-242, hanging drop vapour diffusion method, 0.001 ml of protein solution, containing 20 mM Tris-HCl, 5% v/v glycerol, 0.1 mM TCEP, 100 mM sodium chloride, pH 8.0, is mixed with 0.001 ml of reservoir solution, containing 0.04 M citric acid, 0.06 M Bis-Tris propane, 5% v/v glycerol, 18-21% w/v PEG 3350, and equilibrated against 0.2 ml of reservoir solution, 22°C, 5 days to 2 weeks, X-ray diffraction structure determination and analysis at 2.40 A resolution

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q96GX9	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme residues 20-242 from Escherichia coli strain Rosetta 2(DE3) by metal affinity chhromatography, anion exchange chromatography, gel filtration, and ultrafiltration	Homo sapiens

Synonyms

Synonyms	Comment	Organism
5-methylthioribulose-1-phosphate dehydratase	-	Homo sapiens
Apaf-1-interacting protein	-	Homo sapiens
APIP	-	Homo sapiens
MtnB	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	Apaf-1-interacting protein (APIP) is known to inhibit two different types of cell death: caspase-1-dependent pyroptosis and caspase-9-dependent apoptosis. The enzyme is also involved in the methionine-salvage pathway, where it is called 5-methylthioribulose-1-phosphate dehydratase (MtnB). The enzyme activity seems to be essential for inhibition of pyroptosis by the enzyme, but not for inhibition of apoptosis, overview	Homo sapiens