Any feedback?
Literature summary for 4.2.1.108 extracted from
- Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC (2011), Appl. Microbiol. Biotechnol., 91, 113-122.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged ectoine synthase in Escherichia coli strain BL21(DE3) | Halomonas elongata |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of enzyme-deficient strains by gene ectC inframe deletion though the splicing-by-overlap-extension PCR technique | Halomonas elongata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0287 | - |
L-homoectoine | recombinant enzyme, pH 8.5, 21°C | Halomonas elongata |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16520 | - |
x * 16520, recombinant His-tagged enzyme, SDS-PAGE | Halomonas elongata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N4-acetyl-L-2,4-diaminobutanoate | Halomonas elongata | an intramolecular condensation reaction | L-ectoine + H2O | - |
r | |
| N4-acetyl-L-2,4-diaminobutanoate | Halomonas elongata DSM 2581 | an intramolecular condensation reaction | L-ectoine + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halomonas elongata | - |
gene ectC | - |
| Halomonas elongata DSM 2581 | - |
gene ectC | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged ectoine synthase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Halomonas elongata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
purified recombinant enzyme, substrate is 4,5-dihydro-2-methylimidazole-4-carboxylate, hydrolytic reaction, pH 8.5, 21°C | Halomonas elongata |
| 0.3 | - |
purified recombinant enzyme, substrate is 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, hydrolytic reaction, pH 8.5, 21°C | Halomonas elongata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O | - |
Halomonas elongata | ? | - |
r | |
| D,L-4,5-dihydro-2-methylimidazole-4-carboxylate + H2O | - |
Halomonas elongata DSM 2581 | ? | - |
r | |
| L-glutamine | - |
Halomonas elongata | 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O | - |
r | |
| L-glutamine | - |
Halomonas elongata DSM 2581 | 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate + H2O | - |
r | |
| L-homoectoine + H2O | - |
Halomonas elongata | ? | - |
r | |
| additional information | ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview | Halomonas elongata | ? | - |
? | |
| additional information | ectoine synthase accepts more than one substrate and is a reversible enzyme able to catalyze both the intramolecular condensation into and the hydrolytic cleavage of cyclic amino acid derivatives. It forms 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, ADPC, by cyclic condensation of glutamine in a reversible reaction. A number of ectoine derivatives, in particular 4,5-dihydro-2-methylimidazole-4-carboxylate and L-homoectoine, are also cleaved by ectoine synthase. Proposed reaction mechanism leading to L-ectoine and 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate, respectively, catalyzed by ectoine synthase, overview | Halomonas elongata DSM 2581 | ? | - |
? | |
| N4-acetyl-L-2,4-diaminobutanoate | an intramolecular condensation reaction | Halomonas elongata | L-ectoine + H2O | - |
r | |
| N4-acetyl-L-2,4-diaminobutanoate | an intramolecular condensation reaction | Halomonas elongata | L-ectoine + H2O | i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase | r | |
| N4-acetyl-L-2,4-diaminobutanoate | an intramolecular condensation reaction | Halomonas elongata DSM 2581 | L-ectoine + H2O | - |
r | |
| N4-acetyl-L-2,4-diaminobutanoate | an intramolecular condensation reaction | Halomonas elongata DSM 2581 | L-ectoine + H2O | i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid, over 95% conversion by the recombinant ectoine synthase | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16520, recombinant His-tagged enzyme, SDS-PAGE | Halomonas elongata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EctC | - |
Halomonas elongata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
assay at | Halomonas elongata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Halomonas elongata |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | in Halomonas elongata mutant strains deficient in ectA, encoding L-2,4-diaminobutyric acid acetyltransferase, no ectoine synthesis is obeserved | Halomonas elongata |
| metabolism | ectoine synthase is involved in the biosynthetic pathway of L-ectoine and (S,S)-beta-hydroxyectoine, involvement of ectoine synthase in 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate biosynthesis in vitro | Halomonas elongata |
| physiological function | extraordinary protective properties of 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate produced by ectoine synthase | Halomonas elongata |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
