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Literature summary for 4.2.1.108 extracted from

  • Peters, P.; Galinski, E.A.; Trüper, H.G.
    The biosynthesis of ectoine (1990), FEMS Microbiol. Lett., 71, 157-162.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-N-acetyl-L-2,4-diaminobutanoate Halorhodospira halochloris final step in L-ectoine biosynthesis, pathway overview L-ectoine + H2O
-
r
4-N-acetyl-L-2,4-diaminobutanoate Halomonas elongata final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M L-ectoine + H2O
-
r

Organism

Organism UniProt Comment Textmining
Halomonas elongata
-
extremely halophilic eubacterium, strain DSM 2581
-
Halorhodospira halochloris
-
extremely halophilic eubacterium, strain DSM 1059
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture photosynthetically grown cells Halorhodospira halochloris
-
culture condition:glucose-grown cell
-
Halomonas elongata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview Halorhodospira halochloris L-ectoine + H2O
-
r
4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, pathway overview, L-ectoine is the main compatible solute in the organism with a cytoplasmic concentration of 1-2 M Halomonas elongata L-ectoine + H2O
-
r
4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Halomonas elongata L-ectoine + H2O NMR and thin layer chromatography product identification r
4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Halorhodospira halochloris L-ectoine + H2O NMR and TLC product identification r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Halorhodospira halochloris
37
-
assay at Halomonas elongata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Halorhodospira halochloris
9
-
assay at Halomonas elongata