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Literature summary for 4.2.1.107 extracted from

  • Qin, Y.; Haapalainen, A.M.; Conry, D.; Cuebas, D.A.; Hiltunen, J.K.; Novikov, D.K.
    Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis (1997), Biochem. J., 328, 377-382.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) plysS cells, for analysis the role of the hydratase in the beta-oxidation Rattus norvegicus

General Stability

General Stability Organism
still active after 3 h of incubation in the enzyme assay Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
SDS-PAGE Rattus norvegicus
46580
-
amino acid sequence analysis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 13% yield, anion- and cation exchange chromatography, and gel filtration on Superdex 200 HR Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48
-
recombinant enzyme, pH 7.0 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O the equilibrium lies in the direction of the dehydration Rattus norvegicus (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA only the (24R,25R) diastomer as product, analyzed using reverse-phase HPLC ?

Synonyms

Synonyms Comment Organism
46 kDa hydratase 2
-
Rattus norvegicus
perMFE-2 multifunctional enzyme, cf. EC 4.2.1.119, second multifunctional enzyme in rat liver peroxisome perMFE-1, cf. EC 1.1.1.35 and EC 5.3.3.8 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rattus norvegicus

General Information

General Information Comment Organism
physiological function product of the reaction (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is characterized as a physiological intermediate in bile acid synthesis, in the beta-oxidative side-chain modifications Rattus norvegicus