Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) plysS cells, for analysis the role of the hydratase in the beta-oxidation | Rattus norvegicus |
General Stability | Organism |
---|---|
still active after 3 h of incubation in the enzyme assay | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus norvegicus | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
SDS-PAGE | Rattus norvegicus |
46580 | - |
amino acid sequence analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, 13% yield, anion- and cation exchange chromatography, and gel filtration on Superdex 200 HR | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
48 | - |
recombinant enzyme, pH 7.0 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O | the equilibrium lies in the direction of the dehydration | Rattus norvegicus | (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA | only the (24R,25R) diastomer as product, analyzed using reverse-phase HPLC | ? |
Synonyms | Comment | Organism |
---|---|---|
46 kDa hydratase 2 | - |
Rattus norvegicus |
perMFE-2 | multifunctional enzyme, cf. EC 4.2.1.119, second multifunctional enzyme in rat liver peroxisome perMFE-1, cf. EC 1.1.1.35 and EC 5.3.3.8 | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | product of the reaction (24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is characterized as a physiological intermediate in bile acid synthesis, in the beta-oxidative side-chain modifications | Rattus norvegicus |