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Literature summary for 4.2.1.105 extracted from

  • Shimamura, M.; Akashi, T.; Sakurai, N.; Suzuki, H.; Saito, K.; Shibata, D.; Ayabe, S.; Aoki, T.
    2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus (2007), Plant Cell Physiol., 48, 1652-1657.
    View publication on PubMed
Search for more literature for 4.2.1.105

Activating Compound

Activating Compound Comment Organism Structure
additional information up-regulated by treatment with GSH Lotus japonicus

Application

Application Comment Organism
agriculture regulation of isoflavonoid biosynthesis Lotus japonicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, Agrobacterium rhizogenes strain ATCC15834 used as recipient of the expression vectors, overexpression of heterologous cDNA of 2-hydroxyisoflavanone dehydratase of Glycine maximus yields nine overexpressor lines, overexpressors show poor elongation and branching of root hairs if compared to control plants Lotus japonicus
introduction of soybean HID into the hairy roots of a model legume Lotus japonicus Glycine max

Protein Variants

Protein Variants Comment Organism
additional information transformed plants overexpressing 2-hydroxyisoflavanone dehydratase, studies on regulatory mechanism of isoflavonoid biosynthesis Lotus japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Glycine max 2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus ?
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 ?

Organism

Organism UniProt Comment Textmining
Glycine max
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Lotus japonicus
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 model legume Lotus japonicus (Regel) K. Larsen, hairy root cultures of Lotus japonicus B-129, control plants and transformed plants overexpressing 2-hydroxyisoflavanone dehydratase of Glycine max
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Source Tissue

Source Tissue Comment Organism Textmining
root hairy root cultures of Lotus japonicus B-129 Lotus japonicus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 flavonoid production tested using GSH-treated compared with water-treated and non-treated hairy roots regarding activities of endogenous and overexpressed 2-hydroxyisoflavanone dehydratase, flavonoids obtained from 30-day old hairy roots of different genotypes and analyzed by HPLC Lotus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5,7,4'-tetrahydroxyisoflavanone flavonoid metabolism in hairy root cultures of Lotus japonicus, control plants and plants overexpressing 2-hydroxyisoflavanone Lotus japonicus ? accumulation of daidzein and genistein in overexpressors indicating that 2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity, production of coumestrol in control plants and in plants overexpressing 2-hydroxyisoflavanone dehydratase, production of isoliquiritigenin and liquiritigenin in overexpressors of 2-hydroxyisoflavanone ?
additional information 2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus Glycine max ?
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 ?

Synonyms

Synonyms Comment Organism
2-hydroxyisoflavanone dehydratase
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 Lotus japonicus
GmHID
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 Glycine max
HID
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 Lotus japonicus