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Literature summary for 4.2.1.104 extracted from
- A cyanase is transcriptionally regulated by arginine and involved in cyanate decomposition in Sordaria macrospora (2008), Fungal Genet. Biol., 45, 1458-1469.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bicarbonate | is required for the catalytic activity of the Sordaria macrospora cyanase | Sordaria macrospora |  |
| Cyanate | - |
Sordaria macrospora | |
| additional information | cyn1 transcript levels are strongly down-regulated by the addition of arginine and up-regulated by cyanate. The addition of both, cyanate and arginine, results in a loss of regulation. It seems that arginine and cyanate have an antagonistic effect on the expression of the Sordaria macrospora cyn1 | Sordaria macrospora |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain M15 | Sordaria macrospora |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E107A | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
| E107D | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
| additional information | a delta cyn1 knockout in Sordaria macrospora is totally devoid of cyanase activity and shows an increased sensitivity to exogenously supplied cyanate in an arginine-depleted medium, defects in ascospore germination, but no other obvious morphological phenotype | Sordaria macrospora |
| additional information | Arg104, Glu107 and Ser130 are essential for enzyme activity of the fungal cyanase, irrespectively of substitution by Alanine or another amino-acid | Sordaria macrospora |
| R104A | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
| R104L | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
| S130A | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
| S130T | site-directed mutagenesis, soluble, no activity | Sordaria macrospora |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cyanate | partial auto-inhibition at high concentrations of potassium cyanate | Sordaria macrospora | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
Cyanate | bicarbonate concentration 5 mM | Sordaria macrospora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
SDS-PAGE, monomer calculated: 18100 + His-tag: 600 | Sordaria macrospora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanate + HCO3- + 2 H+ | Sordaria macrospora | - |
NH3 + 2 CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sordaria macrospora | D1ZHN6 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Sordaria macrospora | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyanate + HCO3- + 2 H+ | - |
Sordaria macrospora | NH3 + 2 CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 19000 recombinant enzyme + His-tag | Sordaria macrospora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyanase | - |
Sordaria macrospora |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 80 | quite stable even at high temperatures | Sordaria macrospora |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | 80 | quite stable even at high temperatures | Sordaria macrospora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
potassium phosphate buffer (50 mM), 27°C, 10 min | Sordaria macrospora |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 8.8 | using potassium phosphate buffer (50 mM) the enzyme retains more than 50% activity over a wide pH range after incubation at 27°C for 10 min | Sordaria macrospora |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | 8.8 | using potassium phosphate buffer (50 mM) the enzyme retains more than 50% activity over a wide pH range after incubation at 27°C for 10 min | Sordaria macrospora |
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