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Literature summary for 4.2.1.104 extracted from

  • Espie, G.S.; Jalali, F.; Tong, T.; Zacal, N.J.; So, A.K.
    Involvement of the cynABDS operon and the CO2-concentrating mechanism in the light-dependent transport and metabolism of cyanate by cyanobacteria (2007), J. Bacteriol., 189, 1013-1024.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
molecular biology analyses of conditions to metabolize exogenously supplied cyanate Synechocystis sp.
molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the Cyn-ABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), low internal pools of HCO3- and CO2 result in an insufficient supply of bicarbonate Synechococcus elongatus
molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), inactivation of the cynS gene leads to inability of decomposition of external cyanate Synechococcus elongatus
molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), mutagensis of a periplasmatic binding protein of a multicomponent ABC-transporter (CynA), leads to inability of decomposition of external cyanate due to impaired cyanate uptake, cyanase function is not affected Synechococcus elongatus
molecular biology analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the operon CynABDS, light and internal pools of HCO3- and CO2 Synechococcus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Synechococcus elongatus
expressed in Escherichia coli Synechococcus sp.
expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information targeted mutants of Synechococcus elongatus strain PCC7942 disrupted in the coding sequences of the gene encoding the cytosoliyc cyanase (CynS) to study metabolism of exogenously supplied cyanate Synechococcus elongatus
additional information targeted mutants of Synechococcus elongatus strain PCC7942 impaired in the CO2-concentrating mechanism CCM to study metabolism of exogenously supplied cyanate in cyanobacteria, inactivation of CCM impairs metabolism of external cyanate by insufficient internal pools of HCO3- and CO2 resulting in low levels of the co-substrate bicarbonate that is required for cyanase activity Synechococcus elongatus
additional information targeted mutants of Synechococcus elongatus strain PCC7942, disruption of the cynA gene, encoding a binding protein of a multicomponent ABC-transporter, inactivation of CynA does not affect cyanase function but inhibits decomposition of external cyanate Synechococcus elongatus

Inhibitors

Inhibitors Comment Organism Structure
oxalate the membrane-impermeant inhibitor of cyanobacterial cyanase has minor effects on acid-labile or acid-stable fractions indicating the absence of cyanase from the periplasm Synechococcus elongatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Synechococcus sp. 5829
-
cytosol
-
Synechococcus elongatus 5829
-
cytosol the cytosolic cyanase is encoded by the cynS gene, part of the operon CynABDS Synechococcus elongatus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyanate + HCO3- + 2 H+ Synechococcus sp.
-
NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+ Synechococcus elongatus
-
NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+ Synechococcus elongatus PCC 7942
-
NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+ Synechococcus sp. UTEX625
-
NH3 + 2 CO2
-
?
NCO- + HCO3- + H+ Synechococcus elongatus utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
?
NCO- + HCO3- + H+ Synechococcus sp. utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
?
NCO- + HCO3- + H+ Synechocystis sp. utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
?
NCO- + HCO3- + H+ Synechococcus elongatus utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH4+ + CO2
-
?
NCO- + HCO3- + H+ Synechococcus elongatus PCC 7942 utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH3 + CO2
-
?
NCO- + HCO3- + H+ Synechococcus elongatus PCC 7942 utilization of exogenous cyanate as a niche source of C and N in cyanobacteria NH4+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus
-
strain PCC7942
-
Synechococcus elongatus Q59948 strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon
-
Synechococcus elongatus Q59948 strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase
-
Synechococcus elongatus Q59948 strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM)
-
Synechococcus elongatus PCC 7942
-
strain PCC7942
-
Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon
-
Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase
-
Synechococcus elongatus PCC 7942 Q59948 strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM)
-
Synechococcus sp.
-
-
-
Synechococcus sp. Q5N0J1 strain UTEX625, also known as Synechococcus sp. strain PCC6301
-
Synechococcus sp. UTEX625
-
-
-
Synechocystis sp. Q55367 strain PCC6803
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light Synechocystis sp.
additional information
-
cyanase activity shown in whole-cell extracts of the photoautotrophic Synechococcus sp. strain UTEX 625, ability to utilize cyanate-derived CO2 to support photosynthesis affected Synechococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanate + HCO3- + 2 H+
-
Synechococcus sp. NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+
-
Synechococcus elongatus NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+
-
Synechococcus elongatus PCC 7942 NH3 + 2 CO2
-
?
cyanate + HCO3- + 2 H+
-
Synechococcus sp. UTEX625 NH3 + 2 CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus NH3 + CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus sp. NH3 + CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechocystis sp. NH3 + CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate in cyanobacteria Synechocystis sp. NH3 + CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate in photoautotroph cyanobacteria Synechococcus sp. NH3 + CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus NH3 + CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus PCC 7942 NH3 + CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus PCC 7942 NH3 + CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus NH4+ + CO2
-
?
NCO- + HCO3- + H+ utilization of exogenous cyanate as a niche source of C and N in cyanobacteria Synechococcus elongatus PCC 7942 NH4+ + CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus NH4+ + 2 CO2
-
?
NCO- + HCO3- + H+ studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 Synechococcus elongatus PCC 7942 NH4+ + 2 CO2
-
?

Synonyms

Synonyms Comment Organism
cyanase
-
Synechococcus elongatus
cyanase
-
Synechococcus sp.
cyanase
-
Synechocystis sp.