BRENDA - Enzyme Database
show all sequences of 4.2.1.104

Kinetic properties of cyanase

Anderson, P.M.; Little, R.M.; Biochemistry 25, 1621-1626 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
competitive to bicarbonate
Escherichia coli
Br-
-
Escherichia coli
Cl-
-
Escherichia coli
formate
-
Escherichia coli
malonate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
N3-
competitive to cyanate
Escherichia coli
NO2-
-
Escherichia coli
NO3-
-
Escherichia coli
oxalate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
oxaloacetate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Reaction
Reaction
Commentary
Organism
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2
rapid equilibrium random mechanism
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Cyanate + bicarbonate
-
210678
Escherichia coli
CO2 + carbamate
-
210678
Escherichia coli
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
competitive to bicarbonate
Escherichia coli
Br-
-
Escherichia coli
Cl-
-
Escherichia coli
formate
-
Escherichia coli
malonate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
N3-
competitive to cyanate
Escherichia coli
NO2-
-
Escherichia coli
NO3-
-
Escherichia coli
oxalate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
oxaloacetate
reversible inhibition which can be prevented by saturating concentrations of cyanate or bicarbonate
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Cyanate + bicarbonate
-
210678
Escherichia coli
CO2 + carbamate
-
210678
Escherichia coli
-
Other publictions for EC 4.2.1.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747661
Kebeish
Expression of the cyanobacter ...
Synechocystis sp. PCC 6803, Synechocystis sp. PCC 6803 Kazusa
Environ. Sci. Pollut. Res. Int.
24
11825-11835
2017
-
1
1
-
1
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748012
Schlachter
Structural characterization o ...
Tetranychus urticae
J. Agric. Food Chem.
65
5453-5462
2017
-
1
1
1
-
-
-
-
-
-
2
1
-
4
-
-
1
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
3
3
-
-
-
746656
Butryn
Serendipitous crystallization ...
Serratia proteamaculans, Serratia proteamaculans 568
Acta Crystallogr. Sect. F
71
471-476
2015
-
-
1
1
-
-
-
-
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
1
-
-
-
-
2
3
-
-
-
-
-
-
-
-
-
2
2
-
-
-
748761
Palatinszky
Cyanate as an energy source f ...
Candidatus Nitrososphaera gargensis, Candidatus Nitrososphaera gargensis Ga9.2, Nitrospira moscoviensis, no activity in Nitrosomonas nitrosa strain Nm90
Nature
524
105-108
2015
-
-
1
-
-
-
1
-
-
-
-
3
-
10
-
-
-
-
-
2
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
2
-
-
-
-
-
-
-
-
3
3
-
-
-
729829
Wybouw
A horizontally transferred cya ...
Tetranychus urticae
Insect Biochem. Mol. Biol.
42
881-889
2012
-
-
1
-
-
-
1
1
-
-
-
-
-
7
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
713844
Kamennaya
Characterization of cyanate me ...
Prochlorococcus sp., Prochlorococcus sp. MED4, Synechococcus sp., Synechococcus sp. WH7803
Appl. Environ. Microbiol.
77
291-301
2011
-
-
2
-
-
-
2
-
-
-
1
4
-
10
-
-
1
-
-
-
3
-
4
1
1
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
1
4
-
-
-
1
-
-
3
-
4
1
1
1
1
-
1
-
-
-
-
2
2
-
-
-
716700
Qian
Biochemical and structural pro ...
Arabidopsis thaliana, Arabidopsis thaliana Col 0, Oryza sativa
PLoS ONE
6
e18300
2011
-
-
2
-
3
-
-
-
-
-
2
3
-
10
-
-
2
-
-
1
5
-
3
2
2
2
-
-
2
2
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
2
3
-
-
-
2
-
1
5
-
3
2
2
2
-
-
2
2
-
-
1
3
3
1
-
-
704926
Gupta
Enzymatic mechanism and bioche ...
Pseudomonas pseudoalcaligenes, Pseudomonas putida
J. Hazard. Mater.
176
1-13
2010
-
-
-
-
-
-
-
-
-
-
2
2
-
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
2
-
-
705382
Taiyoji
Identification of proteinaceou ...
Oryza sativa
J. Proteome Res.
8
5165-5174
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
690549
Luque-Almagro
Characterization of the Pseudo ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes CECT 5344
Appl. Environ. Microbiol.
74
6280-6288
2008
3
-
1
-
1
-
5
1
1
-
1
4
-
6
-
-
1
-
-
-
-
-
6
-
1
-
1
-
1
-
-
-
-
-
-
3
-
1
-
-
1
-
-
5
-
1
1
-
1
4
-
-
-
1
-
-
-
-
6
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
692457
Elleuche
A cyanase is transcriptionally ...
Sordaria macrospora
Fungal Genet. Biol.
45
1458-1469
2008
3
-
1
-
7
-
1
1
-
-
1
1
-
4
-
-
-
-
-
1
-
-
1
1
-
1
1
-
1
1
1
-
-
-
-
3
-
1
-
-
7
-
-
1
-
1
-
-
1
1
-
-
-
-
-
1
-
-
1
1
-
1
1
-
1
1
1
-
-
-
-
-
-
-
678457
Bezsudnova
Thiocyanate hydrolase, the pri ...
Thiohalophilus thiocyanatoxydans
Biochim. Biophys. Acta
1774
1563-1570
2007
-
-
-
-
-
-
3
1
-
-
2
1
1
6
-
-
1
-
-
-
3
-
2
1
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
3
3
-
1
-
-
2
1
1
-
-
1
-
-
3
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
680479
Espie
Involvement of the cynABDS ope ...
Synechococcus elongatus, Synechococcus sp., Synechococcus sp. UTEX625, Synechocystis sp.
J. Bacteriol.
189
1013-1024
2007
-
3
3
-
1
-
1
-
5
-
-
7
-
10
-
-
-
-
-
-
2
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
1
-
-
1
-
-
6
-
-
7
-
-
-
-
-
-
2
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681808
Grigoreva
-
Mechanism of cyanide and thioc ...
Pseudomonas putida, Pseudomonas putida 21, Pseudomonas stutzeri, Pseudomonas stutzeri 18
Microbiology
75
266-273
2006
-
2
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
2
4
-
8
-
-
-
-
-
2
4
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
4
-
8
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
660771
Luque-Almagro
Bacterial degradation of cyani ...
Pseudomonas pseudoalcaligenes
Appl. Environ. Microbiol.
71
940-947
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
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1
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1
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-
-
1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
661831
Carepo
Identification of Chromobacter ...
Chromobacterium violaceum
Genet. Mol. Res.
3
181-194
2004
-
1
-
-
-
-
-
-
-
-
-
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4
-
-
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1
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-
-
-
-
-
-
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-
-
-
-
-
-
-
-
653941
Walsh
Structure of cyanase reveals t ...
Escherichia coli
Structure
8
505-514
2000
2
-
-
1
-
-
2
-
-
-
2
-
-
3
-
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651632
Harano
Identification and nitrogen re ...
Synechococcus sp., Synechocystis sp.
J. Bacteriol.
179
5744-5750
1997
4
-
2
-
-
-
2
-
-
-
1
-
-
6
-
-
-
2
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
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-
-
-
-
-
-
210677
Anderson
Formation of intersubunit disu ...
Escherichia coli
J. Biol. Chem.
269
15036-15045
1994
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-
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1
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1
1
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1
1
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-
-
-
-
-
-
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-
-
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210676
Anderson
Reversible dissociation of act ...
Escherichia coli
J. Biol. Chem.
263
5674-5680
1988
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5
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1
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2
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1
1
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5
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1
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1
1
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-
-
-
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210674
Johnson
Bicarbonate is a recycling sub ...
Escherichia coli
J. Biol. Chem.
262
9021-9025
1987
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-
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2
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1
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1
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1
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210675
Guilloton
Isolation and characterization ...
Escherichia coli
J. Gen. Microbiol.
133
645-653
1987
-
-
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1
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4
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-
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2
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1
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1
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2
-
1
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-
-
-
-
-
-
-
-
-
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-
-
210679
Little
Structural properties of cyana ...
Escherichia coli
J. Biol. Chem.
262
10120-10126
1987
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-
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1
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-
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2
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1
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1
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1
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1
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1
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210680
Kim
Preliminary X-ray crystallogra ...
Escherichia coli
J. Mol. Biol.
198
137-138
1987
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1
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1
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2
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1
1
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1
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1
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1
1
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-
-
-
-
-
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-
-
-
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210681
Sung
Identification, mapping, and c ...
Escherichia coli
J. Bacteriol.
169
2639-2642
1987
-
-
1
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-
-
-
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1
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3
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2
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1
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-
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-
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-
1
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-
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-
2
-
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-
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-
210682
Anderson
Interaction of mono- and diani ...
Escherichia coli
Biochemistry
26
3938-3943
1987
-
-
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-
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-
17
-
-
-
-
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-
3
-
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-
1
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1
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-
17
-
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-
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-
-
-
1
-
-
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-
-
-
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-
210678
Anderson
Kinetic properties of cyanase ...
Escherichia coli
Biochemistry
25
1621-1626
1986
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-
-
-
-
-
10
-
-
-
-
-
-
2
-
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-
1
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1
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-
-
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10
-
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-
-
1
-
-
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-
-
-
-
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210683
Chin
The amino acid sequence of Esc ...
Escherichia coli
J. Biol. Chem.
258
276-282
1983
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-
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-
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-
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1
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2
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1
1
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1
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1
1
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210673
Anderson
Purification and properties of ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochemistry
19
2882-2888
1980
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-
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-
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1
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3
-
-
178
-
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1
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2
1
1
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1
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1
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-
3
-
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1
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-
2
1
1
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1
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210672
Taussig
The synthesis of the induced e ...
Escherichia coli, Escherichia coli B / ATCC 11303
Biochim. Biophys. Acta
44
510-519
1960
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1
1
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2
-
177
-
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1
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1
1
4
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1
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1
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1
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1
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2
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1
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1
1
4
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1
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1
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