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Literature summary for 4.2.1.10 extracted from

  • Cheung, V.W.; Xue, B.; Hernandez-Valladares, M.; Go, M.K.; Tung, A.; Aguda, A.H.; Robinson, R.C.; Yew, W.S.
    Identification of polyketide inhibitors targeting 3-dehydroquinate dehydratase in the shikimate pathway of Enterococcus faecalis (2014), PLoS ONE, 9, e103598 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.2 A resolution Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
7-hydroxycoumarin uncompetitive inhibitor Enterococcus faecalis
marein flavonoid polyketide, mixed type inhibitor, both inhibits DHQase and retards the growth of Enterococcus faecalis Enterococcus faecalis
rhoifolin flavonoid glycoside, uncompetitive inhibitor Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis P36923
-
-
Enterococcus faecalis ATCC 700802 P36923
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroquinate
-
 Enterococcus faecalis 3-dehydroshikimate + H2O
-
 ?
3-dehydroquinate
-
 Enterococcus faecalis ATCC 700802 3-dehydroshikimate + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
110
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.019
-
 rhoifolin pH 7.5, 25°C Enterococcus faecalis
0.042
-
 7-hydroxycoumarin pH 7.5, 25°C Enterococcus faecalis
0.34
-
 marein pH 7.5, 25°C Enterococcus faecalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1700
-
 3-dehydroquinate pH 7.5, 25°C Enterococcus faecalis