Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.10 extracted from

  • Coderch, C.; Lence, E.; Peon, A.; Lamb, H.; Hawkins, A.R.; Gago, F.; Gonzalez-Bello, C.
    Mechanistic insight into the reaction catalysed by bacterial type II dehydroquinases (2014), Biochem. J., 458, 547-557.
    View publication on PubMed
Search for more literature for 4.2.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
D88N the mutant shows severely reduced activity compared to the wild type enzyme and is not active at pH 7.0 but pH 8.0 Mycobacterium tuberculosis
N12S the mutant shows severely reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0114
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
0.0146
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
0.1776
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydroquinate Mycobacterium tuberculosis
-
 3-dehydroshikimate + H2O
-
 ?
3-dehydroquinate Helicobacter pylori
-
 3-dehydroshikimate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Mycobacterium tuberculosis
Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid
-
 Mycobacterium tuberculosis ?
-
 ?
(2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid
-
 Helicobacter pylori ?
-
 ?
3-dehydroquinate
-
 Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
 ?
3-dehydroquinate
-
 Helicobacter pylori 3-dehydroshikimate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DHQ2
-
 Mycobacterium tuberculosis
DHQ2
-
 Helicobacter pylori
Type II dehydroquinase
-
 Mycobacterium tuberculosis
Type II dehydroquinase
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis
0.0065
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
0.1542
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis
0.6
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
10.5
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis