Literature summary for 4.2.1.10 extracted from

Devi, A.S.; Ebihara, A.; Kuramitsu, S.; Yokoyama, S.; Kumarevel, T.; Ponnuraj, K.
Crystal structure of type I 3-dehydroquinate dehydratase of Aquifex aeolicus suggests closing of active site flap is not essential for enzyme action (2013), Biochem. Biophys. Res. Commun., 432, 350-354.

Search for more literature for 4.2.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
the enzyme is co-crystallized with the ligand TLA(C4H6O6) or L(+)-tartaric acid, sitting drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate and 0.1 M citrate, pH 5.6	Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	2 * 27000, SDS-PAGE	Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-dehydroquinate	Aquifex aeolicus	-	3-dehydroshikimate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
SuperQ Toyopearl 650 column chromatography, Resource Q column chromatography, hydroxylapatite column chromatography, and Superdex 200 gel filtration	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-dehydroquinate	-	Aquifex aeolicus	3-dehydroshikimate + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 27000, SDS-PAGE	Aquifex aeolicus

Synonyms

Synonyms	Comment	Organism
3-dehydroquinase	-	Aquifex aeolicus
3-dehydroquinate dehydratase	-	Aquifex aeolicus
3-DHQase	-	Aquifex aeolicus
type I 3-dehydroquinate dehydratase	-	Aquifex aeolicus

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Release 2023.1 (January 2023)