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Literature summary for 4.2.1.10 extracted from
- Cloning, sequencing, expression, purification and preliminary characterization of a type II dehydroquinase from Helicobacter pylori (1996), Biochem. J., 319, 559-565.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Helicobacter pylori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
3-dehydroquinate | - |
Helicobacter pylori |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18483 | - |
12 * 18483, calculation from nucleotide sequence | Helicobacter pylori |
| 230000 | - |
gel filtration | Helicobacter pylori |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroquinate | Helicobacter pylori | the enzyme is likely to function in the shikimate pathway | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helicobacter pylori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.92 | - |
- |
Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroquinate | - |
Helicobacter pylori | 3-dehydroshikimate + H2O | - |
? | |
| 3-dehydroquinate | the enzyme is likely to function in the shikimate pathway | Helicobacter pylori | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | 12 * 18483, calculation from nucleotide sequence | Helicobacter pylori |
| dodecamer | 12 * 16000-18000, SDS-PAGE | Helicobacter pylori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
3-dehydroquinate | - |
Helicobacter pylori | |
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